Evidence map›Paper›PMID 35519041›Full record

ArticleRSC advances2020

Development of a horseradish peroxidase-nanobody fusion protein for visual detection of ochratoxin A by dot immunoassay.

Qi Chen, Yuanyuan Wang, Fujing Mao, Benchao Su, Kunlu Bao, Zeling Zhang, Guifang Xie, Xing Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Qi ChenCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn wangyuanyuan@hainanu.edu.cn maofujing11@163.com benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn zhangzeling@hainanu.edu.cn xgf@hainanu.edu.cn xliu@hainanu.edu.cn.
Yuanyuan WangCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn wangyuanyuan@hainanu.edu.cn maofujing11@163.com benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn zhangzeling@hainanu.edu.cn xgf@hainanu.edu.cn xliu@hainanu.edu.cn.
Fujing MaoCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn wangyuanyuan@hainanu.edu.cn maofujing11@163.com benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn zhangzeling@hainanu.edu.cn xgf@hainanu.edu.cn xliu@hainanu.edu.cn.
Benchao SuCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn wangyuanyuan@hainanu.edu.cn maofujing11@163.com benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn zhangzeling@hainanu.edu.cn xgf@hainanu.edu.cn xliu@hainanu.edu.cn.
Kunlu BaoCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn wangyuanyuan@hainanu.edu.cn maofujing11@163.com benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn zhangzeling@hainanu.edu.cn xgf@hainanu.edu.cn xliu@hainanu.edu.cn.
Zeling ZhangCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn wangyuanyuan@hainanu.edu.cn maofujing11@163.com benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn zhangzeling@hainanu.edu.cn xgf@hainanu.edu.cn xliu@hainanu.edu.cn.
Guifang XieCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn wangyuanyuan@hainanu.edu.cn maofujing11@163.com benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn zhangzeling@hainanu.edu.cn xgf@hainanu.edu.cn xliu@hainanu.edu.cn.
Xing LiuCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn wangyuanyuan@hainanu.edu.cn maofujing11@163.com benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn zhangzeling@hainanu.edu.cn xgf@hainanu.edu.cn xliu@hainanu.edu.cn.ORCID https://orcid.org/0000-0002-1301-4837
Hainan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ochratoxin A (OTA) is a common cereal mycotoxin that seriously threatens food safety and public health. Herein a horseradish peroxidase-nanobody fusion protein (HRP-Nb) retaining antibody and enzyme activity was obtained after inclusion body denaturation and renaturation and enzyme reconstitution, which served both as the primary antibody and reporter enzyme and was applied to develop a membrane-based dot immunoassay (HN-DIA) for OTA visual detection. Based on the optimal experimental conditions, the HN-DIA could be finished in 10 min with a cut-off limit of 50 μg kg

Identifiers

PMID35519041
PMCPMC9056725
OpenAlexW3085261261

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.