Evidence map›Paper›PMID 35517360›Full record

ArticleRSC advances2020

Development of a nanobody tagged with streptavidin-binding peptide and its application in a Luminex fluoroimmunoassay for alpha fetal protein in serum.

Qi Chen, Danyang Sun, Hua Pei, Benchao Su, Kunlu Bao, Hongmei Cao, Chenghui Zhang, Bruce D Hammock, Xing Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Nanobody based immunoassay for alpha fetal protein detection using streptavidin-conjugated polymerized horseradish peroxidase for signal amplification.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

Qi ChenCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn sdy@hainanu.edu.cn benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn hmcao@hainanu.edu.cn zchlm@163.com xliu@hainanu.edu.cn.
Danyang SunCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn sdy@hainanu.edu.cn benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn hmcao@hainanu.edu.cn zchlm@163.com xliu@hainanu.edu.cn.
Hua PeiDepartment of Clinical Laboratory, The Second Affiliated Hospital of Hainan Medical University Haikou 570311 China phzmh61@aliyun.com.
Benchao SuCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn sdy@hainanu.edu.cn benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn hmcao@hainanu.edu.cn zchlm@163.com xliu@hainanu.edu.cn.
Kunlu BaoCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn sdy@hainanu.edu.cn benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn hmcao@hainanu.edu.cn zchlm@163.com xliu@hainanu.edu.cn.
Hongmei CaoCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn sdy@hainanu.edu.cn benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn hmcao@hainanu.edu.cn zchlm@163.com xliu@hainanu.edu.cn.
Chenghui ZhangCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn sdy@hainanu.edu.cn benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn hmcao@hainanu.edu.cn zchlm@163.com xliu@hainanu.edu.cn.
Bruce D HammockDepartment of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California Davis California 95616 USA bdhammock@ucd.edu.
Xing LiuCollege of Food Science and Engineering, Hainan University Haikou 570228 China qichen@hainanu.edu.cn sdy@hainanu.edu.cn benchao312@hainanu.edu.cn baokunlu@hainanu.edu.cn hmcao@hainanu.edu.cn zchlm@163.com xliu@hainanu.edu.cn.ORCID https://orcid.org/0000-0002-1301-4837
Hainan University · CNHaikou City People's Hospital · CNUniversity of California System · US

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI HAMMOCK, BRUCE D · 1987 to 2021
$50.1M
Supplement for bioactive lipids as effectors and indicators of the deleterious effects of human healthR35ES030443 · NIEHS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MORISSEAU, CHRISTOPHE HP · 2019 to 2025
$5.8M
NIEHS NIH HHS P42 ES004699NIEHS NIH HHS R35 ES030443
6 · The paper itself

Abstract

Sensitive and accurate detection of disease-related biomarkers can promote the early screening and diagnosis of cancers for improving the prognosis and survival of patients. Herein alpha fetal protein (AFP) was selected as the model macromolecule antigen and we developed AFP-specific alpaca nanobodies (Nbs) from an immunized phage-displayed Nb library. Then Nbs tagged with streptavidin-binding peptide (Nb-SBP) were constructed and used to develop an Nb-SBP-mediated fluoroimmunoassay based on the Luminex-200 system (NS-LFIA). Based on the optimal experimental conditions, the NS-LFIA has a limit of detection of 0.237 ng mL

Identifiers

PMID35517360
PMCPMC9054930
OpenAlexW3036414432

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.