Evidence map›Paper›PMID 35516001›Full record

ArticleData in brief2022

Dataset of high-throughput ligand screening against the RNA Packaging Signals regulating Hepatitis B Virus nucleocapsid formation.

Fardokht Abulwerdi, Farzad Fatehi, Iain W Manfield, Stuart F J Le Grice, John S Schneekloth, Reidun Twarock, Peter G Stockley, Nikesh Patel

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Data in brief, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Fardokht AbulwerdiCenter for Cancer Research, National Cancer Institute, 21702-1201, Frederick, MD, USA.
Farzad FatehiDepartment of Mathematics, University of York, York, YO10 5DD, UK.
Iain W ManfieldAstbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
Stuart F J Le GriceCenter for Cancer Research, National Cancer Institute, 21702-1201, Frederick, MD, USA.
John S SchneeklothCenter for Cancer Research, National Cancer Institute, 21702-1201, Frederick, MD, USA.
Reidun TwarockDepartment of Mathematics, University of York, York, YO10 5DD, UK.
Peter G StockleyAstbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
Nikesh PatelAstbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.
National Cancer Institute · USUniversity of Leeds · GBUniversity of York · GB

Funding

Identification of Nucleic Acid Binding Small MoleculesZIABC011585 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI SCHNEEKLOTH, JOHN · 2014 to 2025
$18.6M
Medical Research Council MR/N021517/1MRF_ MRF-044-0002-RG-PATELWellcome Trust 110145/Z/15/Z
6 · The paper itself

Abstract

Multiple ssRNA viruses which infect bacteria, plants or humans use RNA Packaging Signal (PS)-mediated regulation during assembly to package their genomes faithfully and efficiently. PSs typically comprise short nucleotide recognition motifs, most often presented in the unpaired region of RNA stem-loops, and often bind their cognate coat proteins (CPs) with nanomolar affinity. PSs identified to date are resilient in the face of the typical error prone replication of their virus-coded polymerases, making them potential drug targets. An immobilised array of small molecular weight, drug-like compounds was panned against a fluorescently-labelled oligonucleotide encompassing the most conserved Hepatitis B Virus (HBV) PS, PS1, known to be a major determinant in nucleocapsid formation. This identified > 70 compounds that bind PS1 uniquely in the array. The commercially available 66 of these were tested for their potential effect(s) on HBV nucleocapsid-like particle (NCP) assembly

Indexed as

Assembly inhibitorsHepatitis B Virus nucleocapsid assemblyHigh-throughput screeningRNA Packaging Signal-mediated virus assembly

Identifiers

PMID35516001
PMCPMC9065705
OpenAlexW4224440629

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.