ArticlePlant biotechnology journal2022
Custom-made design of metabolite composition in N. benthamiana leaves using CRISPR activators.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
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- Epigenome Editing for Cannabinoid Yield: Targets, Tools, and Priorities inInternational journal of molecular sciences · 2026Review
- dSaCas9 enables enhanced transcriptional activation in Nicotiana benthamiana compared to its dSpCas9 ortholog.BMC plant biology · 2026Article
- Engineering Conditional Transgene Expression in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- Turbo-charging crop improvement: harnessing multiplex editing for polygenic trait engineering and beyond.The Plant journal : for cell and molecular biology · 2025Review
- Cascade Oxygen Control Enhances Growth ofPlants (Basel, Switzerland) · 2025Article
- Programmable genome engineering and gene modifications for plant biodesign.Plant communications · 2025Review
- Efficient, cell-type-specific production of flavonols by multiplexed CRISPR activation of a suite of metabolic enzymes.Nature communications · 2025Article
- Small DNA elements can act as both insulators and silencers in plants.The Plant cell · 2025Article
- Molecular mechanisms driving the unusual pigmentation shift during eggplant fruit development.Plant communications · 2025Article
- A hybrid pathway for self-sustained luminescence.Science advances · 2024Article
- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
- From qualitative to quantitative: the state of the art and challenges for plant synthetic biology.Quantitative biology (Beijing, China) · 2023Review
- Efficient gene activation in plants by the MoonTag programmable transcriptional activator.Nucleic acids research · 2023Article
- The design of synthetic gene circuits in plants: new components, old challenges.Journal of experimental botany · 2023Review
- Efficient gene activation in plants by the MoonTag programmable transcriptional activator.bioRxiv : the preprint server for biology · 2023Article
- FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi.Frontiers in bioengineering and biotechnology · 2023Article
- The advent of plant cells in bioreactors.Frontiers in plant science · 2023Review
- Effects of Poty-Potexvirus Synergism on Growth, Photosynthesis and Metabolite Status ofViruses · 2022Article
- Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional regulators based on CRISPR architecture expand our ability to reprogramme endogenous gene expression in plants. One of their potential applications is the customization of plant metabolome through the activation of selected enzymes in a given metabolic pathway. Using the previously described multiplexable CRISPR activator dCasEV2.1, we assayed the selective enrichment in Nicotiana benthamiana leaves of four different flavonoids, namely, naringenin, eriodictyol, kaempferol, and quercetin. After careful selection of target genes and guide RNAs combinations, we created successful activation programmes for each of the four metabolites, each programme activating between three and seven genes, and with individual gene activation levels ranging from 4- to 1500-fold. Metabolic analysis of the flavonoid profiles of each multigene activation programme showed a sharp and selective enrichment of the intended metabolites and their glycosylated derivatives. Remarkably, principal component analysis of untargeted metabolic profiles clearly separated samples according to their activation treatment, and hierarchical clustering separated the samples into five groups, corresponding to the expected four highly enriched metabolite groups, plus an un-activated control. These results demonstrate that dCasEV2.1 is a powerful tool for re-routing metabolic fluxes towards the accumulation of metabolites of interest, opening the door for the custom-made design of metabolic contents in plants.
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