Evidence map›Paper›PMID 35513844›Full record

ArticleReproductive biology and endocrinology : RB&E2022

Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis.

Xi Wang, Mengmeng Zhang, Liaofei Jiang, Xiaoling Fang, Tingting Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive biology and endocrinology : RB&E, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 2 pooled it
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 2 syntheses or guidelines pooled it, 19 citations in OpenAlex.

  1. Pooled it
  2. Roles of AFAP1-AS1 in Gynecology and Urogenital System.Current pharmaceutical design · 2024
    Pooled it
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. International journal of genomics · 2022
    Article
  11. Natural antisense transcripts as drug targets.Frontiers in molecular biosciences · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Xi WangDepartment of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, NO.139 Renmin Road, Changsha, Hunan, 410000, PR China.
Mengmeng ZhangDepartment of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, NO.139 Renmin Road, Changsha, Hunan, 410000, PR China.
Liaofei JiangDepartment of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, NO.139 Renmin Road, Changsha, Hunan, 410000, PR China.
Xiaoling FangDepartment of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, NO.139 Renmin Road, Changsha, Hunan, 410000, PR China.
Tingting ZhangDepartment of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, NO.139 Renmin Road, Changsha, Hunan, 410000, PR China. zhangtingting823@csu.edu.cn.
Second Xiangya Hospital of Central South University · CNCentral South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEndometriosis (EMS) remains a major challenge to reproductive health due to multifactorial etiology, disease heterogeneity, and the lack of appropriate diagnostic markers and treatment. Eexosome (Exo) has become a major factor in progression of a variety of diseases. However, the mechanisms directing their role in the pathophysiology of EMS are ill-defined. Here, we aimed to investigate the clinical implications of actin filament associated protein 1-Antisense RNA 1 (AFAP1-AS1) in EMS.

methodsBioinformatics analysis was used to predict the expression and interaction of AFAP1-AS1, miR-15a-5p and BCL9 in EMS, and dual luciferase reporter assay was used to verify the targeted relationship of AFAP1-AS1, miR-15a-5p, and BCL9. The Exo from endometrial stromal cells (ESCs) was isolated and characterized by transmission electron microscopy (TEM) and Nanoparticle tracking analysis (NTA). Exosome uptake studies were performed. For in vitro assay, ectopic ESCs (EcESCs) proliferation, migration, and invasion were assessed by CCK-8 and Transwell assays. In vivo assay was performed by establishment of EMS mice to validate the result derived from in vitro assay.

resultsThe Exo was successfully isolated from ESCs and we observed high expression of AFAP1-AS1 and BCL9 but low expression of miR-15a-5p in EMS. Moreover, Exo derived from EcESCs could deliver AFAP1-AS1 to EcESCs and thus promoting proliferation, migration, and invasion of ESCs. AFAP1-AS1 bound to BCL9, which was targeted by miR-15a-5p in EMS. In vivo experiments in nude mice revealed that inhibition of Exosomal AFAP1-AS1 suppressed migration and invasion of EcESCs through miR-15a-5p/BCL9.

conclusionsCollectively, these findings suggested that ESCs-derived Exo carrying AFAP1-AS1 contributed to EMS pathogenesis. This study might help us realize the etiology of EMS and improve the treatment of the related complications.

Indexed as

EndometriosisMicroRNAsRNA, Long NoncodingAnimalsCell Line, TumorCell MovementCell ProliferationFemaleHumansMiceMice, NudeStromal CellsTranscription FactorsBCL9 protein, humanMicroRNAsMirn15a microRNA, mouseRNA, Long NoncodingTranscription FactorsB-cell lymphoma 9Endometrial stromal cellsEndometriosisExosomeLong non-coding RNA actin filament associated protein 1-antisense RNA 1microRNA-15a-5pProliferation

Identifiers

PMID35513844
PMCPMC9069797
OpenAlexW4229018373

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.