Evidence map›Paper›PMID 35513782›Full record

ArticleBMC plant biology2022

RAD-seq as an effective strategy for heterogenous variety identification in plants-a case study in Italian Ryegrass (Lolium multiflorum).

Qingqing Yu, Yao Ling, Yanli Xiong, Wenda Zhao, Yi Xiong, Zhixiao Dong, Jian Yang, Junming Zhao, Xinquan Zhang, Xiao Ma

Open access · goldAbstract read
In one paragraph

Article in BMC plant biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
8.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 22 citations in OpenAlex.

  1. Genomic insights intoFrontiers in plant science · 2026
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  4. Genetic and genomic resources for turfgrasses: status, applications, and prospects.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Review
  5. Review
  6. Harnessing Genomics for BreedingInternational journal of molecular sciences · 2025
    Article
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  8. Article
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  12. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Qingqing Yu *College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Yao Ling *College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Yanli XiongCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Wenda ZhaoCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Yi XiongCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Zhixiao DongCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Jian YangCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Junming ZhaoCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
Xinquan ZhangCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China. zhangxq@sicau.edu.cn.
Xiao MaCollege of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China. maroar@126.com.
Sichuan Agricultural University · CN

Funding

grants from Sichuan Provincial Plant Breeding Program for Forage herbage in the 14th Five-Year Plan period 2021ZDYF2201Sichuan Provincial Science and technology support program 2019YFN0170
6 · The paper itself

Abstract

The primary approach for variety distinction in Italian ryegrass is currently the DUS (distinctness, uniformity and stability) test based on phenotypic traits. Considering the diverse genetic background within the population and the complexity of the environment, however, it is challenging to accurately distinguish varieties based on DUS criteria alone. In this study, we proposed the application of high-throughput RAD-seq to distinguish 11 Italian ryegrass varieties with three bulks of 50 individuals per variety. Our findings revealed significant differences among the 11 tested varieties. The PCA, DAPC and STRUCTURE analysis indicated a heterogeneous genetic background for all of them, and the AMOVA analysis also showed large genetic variance among these varieties (Φ

Indexed as

LoliumItalyPhenotypePhylogenyLolium multiflorumPhylogenetic analysisRAD-seqSNP markersVariety identification

Identifiers

PMID35513782
PMCPMC9069751
OpenAlexW4228998305

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.