ArticlePlant physiology2022
Integrating GWAS and TWAS to elucidate the genetic architecture of maize leaf cuticular conductance.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Multi-omics revolution to promote plant breeding efficiency.Frontiers in plant science · 2022Pooled it
- Transcriptional control of cuticle formation during development.Journal of experimental botany · 2026Review
- High-Throughput Screen of NPQ in Sorghum Shows Highly Polygenic Architecture of Photoprotection.Plant-environment interactions (Hoboken, N.J.) · 2026Article
- Bridging scales: integrated multi-omics and deep phenotyping for climate resilience in crop plants.Frontiers in plant science · 2026Review
- Genome-Wide Association Study Dissects the Genetic Architecture of Pericarp Traits in Fresh-Eating Maize.Plants (Basel, Switzerland) · 2025Article
- Comprehensive analysis of 1,771 transcriptomes from 7 tissues enhance genetic and biological interpretations of maize complex traits.G3 (Bethesda, Md.) · 2025Article
- In silico prediction of variant effects: promises and limitations for precision plant breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Review
- Cuticle development and the underlying transcriptome-metabolome associations during early seedling establishment.Journal of experimental botany · 2024Article
- Integrative multi-omic analysis identifies genes associated with cuticular wax biogenesis in adult maize leaves.G3 (Bethesda, Md.) · 2024Article
- Genome-wide association analysis and transgenic characterization for amylose content regulating gene in tuber of Dioscorea zingiberensis.BMC plant biology · 2024Article
- Molecular markers and molecular basis of plant type related traits in maize.Frontiers in genetics · 2024Article
- Effects of Climate Change and Drought Tolerance on Maize Growth.Plants (Basel, Switzerland) · 2023Review
- Genome-wide association study and genetic mapping of BhWAX conferring mature fruit cuticular wax in wax gourd.BMC plant biology · 2022Article
- Cellular Protein Trafficking: A New Player in Low-Temperature Response Pathway.Plants (Basel, Switzerland) · 2022Review
- Integration of multi-omics technologies for crop improvement: Status and prospects.Frontiers in bioinformatics · 2022Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cuticle, a hydrophobic layer of cutin and waxes synthesized by plant epidermal cells, is the major barrier to water loss when stomata are closed. Dissecting the genetic architecture of natural variation for maize (Zea mays L.) leaf cuticular conductance (gc) is important for identifying genes relevant to improving crop productivity in drought-prone environments. To this end, we performed an integrated genome- and transcriptome-wide association studies (GWAS and TWAS) to identify candidate genes putatively regulating variation in leaf gc. Of the 22 plausible candidate genes identified, 4 were predicted to be involved in cuticle precursor biosynthesis and export, 2 in cell wall modification, 9 in intracellular membrane trafficking, and 7 in the regulation of cuticle development. A gene encoding an INCREASED SALT TOLERANCE1-LIKE1 (ISTL1) protein putatively involved in intracellular protein and membrane trafficking was identified in GWAS and TWAS as the strongest candidate causal gene. A set of maize nested near-isogenic lines that harbor the ISTL1 genomic region from eight donor parents were evaluated for gc, confirming the association between gc and ISTL1 in a haplotype-based association analysis. The findings of this study provide insights into the role of regulatory variation in the development of the maize leaf cuticle and will ultimately assist breeders to develop drought-tolerant maize for target environments.
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