Evidence map›Paper›PMID 35511227›Full record

ArticleEnvironmental health perspectives2022

Role of OCT3 and DRP1 in the Transport of Paraquat in Astrocytes: A Mouse Study.

Sida Han, Yiwei Feng, Min Guo, Yining Hao, Jian Sun, Yichen Zhao, Qiang Dong, Yanxin Zhao, Mei Cui

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environmental health perspectives, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Sida HanDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Yiwei FengDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Min GuoDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Yining HaoDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Jian SunDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.ORCID 0000-0001-5980-7114
Yichen ZhaoDepartment of Neurology, Tenth People's Hospital, Tongji University, Shanghai, China.
Qiang DongDepartment of Neurology, Huashan Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, China.
Yanxin ZhaoDepartment of Neurology, Tenth People's Hospital, Tongji University, Shanghai, China.
Mei CuiDepartment of Neurology, Huashan Hospital, Fudan University, Shanghai, China.
Fudan University · CNTongji University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundParaquat (PQ) is a pesticide, exposure to which has been associated with an increased risk of Parkinson's disease; however, PQ transport mechanisms in the brain are still unclear. Our previous studies indicated that the organic cation transporter 3 (OCT3) expressed on astrocytes could uptake PQ and protect the dopaminergic (DA) neurons from a higher level of extracellular PQ. At present, it is unknown how OCT3 levels are altered during chronic PQ exposure or aging, nor is it clear how the compensatory mechanisms are triggered by OCT3 deficiency. Dynamic related protein 1 (DRP1) was previously reported to ameliorate the loss of neurons during Parkinson's disease. Nowadays, mounting studies have revealed the functions of astrocyte DRP1, prompting us to hypothesize that DRP1 could regulate the PQ transport capacity of astrocytes.

objectivesThe present study aimed to further explore PQ transport mechanisms in the nigrostriatal system and identify pathways involved in extracellular PQ clearance.

methodsModels of PQ-induced neurodegeneration were established by intraperitoneal (i.p.) injection of PQ in wild-type (WT) and organic cation transporter-3-deficient (

resultsOlder mice and those chronically exposed to PQ had a lower expression of brain OCT3 and, following exposure to a DISCUSSION: The present study mainly focused on the transport mechanisms of PQ between the dopaminergic neurons and astrocytes. Lower OCT3 levels were found in the older or chronically PQ-treated mice. Astrocytes with DRP1 inhibition (by viral tools or mitochondrial division inhibitor-1) had higher levels of ASCT2, which we hypothesize served as an alternative transporter to remove extracellular PQ when OCT3 was deficient. In summary, our data suggest that OCT3, ASCT2 located on astrocytes and the dopamine transporter located on DA terminals may function in a concerted manner to mediate striatal DA terminal damage in PQ-induced neurotoxicity. https://doi.org/10.1289/EHP9505.

Indexed as

Neurotoxicity SyndromesParkinson DiseaseAnimalsAstrocytesCationsDopamine Plasma Membrane Transport ProteinsDopaminergic NeuronsDynaminsMiceOctamer Transcription Factor-3ParaquatRNA, Small InterferingSubstantia NigraCationsDnm1l protein, mouseDopamine Plasma Membrane Transport ProteinsDynaminsOctamer Transcription Factor-3ParaquatPou5f1 protein, mouseRNA, Small Interfering

Identifiers

PMID35511227
PMCPMC9070608
OpenAlexW4229041834

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.