Evidence map›Paper›PMID 35511048›Full record

ArticleEndocrinology2022

Nuclear Progestin Receptor-mediated Linkage of Blood Coagulation and Ovulation.

Jing Huang, Chao Sun, Dong Teng Liu, Nan Nan Zhao, Jordan A Shavit, Yong Zhu, Shi Xi Chen

Erratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Jing HuangState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian361102, China.ORCID 0000-0001-5482-8792
Chao SunState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian361102, China.ORCID 0000-0001-9259-0738
Dong Teng LiuState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian361102, China.
Nan Nan ZhaoState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian361102, China.ORCID 0000-0002-1380-3617
Jordan A ShavitDepartments of Pediatrics and Human Genetics, University of Michigan, Ann Arbor, Michigan 48109, USA.ORCID 0000-0002-2874-4904
Yong ZhuState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian361102, China.ORCID 0000-0003-0374-485X
Shi Xi ChenState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, Fujian361102, China.ORCID 0000-0003-1627-5378
Xiamen University · CNEast Carolina University · USUniversity of Michigan · US

Funding

Genetic and therapeutic studies of hemostatic and thrombotic disorders using zebrafishR35HL150784 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Jordan A. Shavit · 2020 to 2026
$5.4M
Dissection of the mechanisms underlying sex-influenced cardiovascular diseaseR01ES032255 · NIEHS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SHAVIT, JORDAN A · 2020 to 2023
$2.1M
To characterize nongenomic progestin receptors via knockouts in zebrafishR15GM100461 · NIGMS · EAST CAROLINA UNIVERSITY · PI ZHU, YONG · 2013 to 2017
$757k
NHLBI NIH HHS R35 HL150784NIEHS NIH HHS R01 ES032255NIGMS NIH HHS R15 GM100461
6 · The paper itself

Abstract

Ovulation is a dramatic remodeling process that includes rupture of blood capillaries and clotting, but coagulation is not thought to directly regulate this process. Herein, we report remarkable increases of coagulation factors V (f5, ~3145-fold) and tissue factor (f3a, ~120-fold) in zebrafish ovarian follicle cells during ovulation. This increase was mediated through the nuclear progestin receptor (Pgr), which is essential for ovulation in zebrafish, and was totally abolished in ovarian follicular cells from pgr-/- mutants. In addition, promoter activities of f5 and f3a were significantly enhanced by progestin (DHP) via Pgr. Similar regulation of human F5 promoter activity was induced via human PGRB, suggesting a conserved mechanism. Site-directed mutagenesis of the zebrafish f5 promoter further demonstrated a direct regulation of coagulation factors via progestin response elements. Moreover, a stark increase of erythrocytes occurred in capillaries meshed in wild-type preovulatory follicles but was absent in pgr-/- mutants. Interestingly, anticoagulants significantly inhibited ovulation both in vitro and in vivo, respectively. Furthermore, reduced fecundity was observed in f5+/- female zebrafish. Taken together, our study provides plausible evidence for steroid regulation of coagulation factors, and a new hypothesis for blood clotting-triggered ovulation in vertebrates.

Indexed as

ProgestinsReceptors, ProgesteroneAnimalsBlood CoagulationFemaleOvulationSteroidsZebrafishProgestinsReceptors, ProgesteroneSteroidscoagulation factorovulationPgrprogestin

Identifiers

PMID35511048
PMCPMC9653010
OpenAlexW4229020894

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.