Evidence map›Paper›PMID 35510730›Full record

SynthesisMolecular ecology resources2022

Systematic review and meta-analysis: Water type and temperature affect environmental DNA decay.

Philip D Lamb, Vera G Fonseca, David L Maxwell, Chibuzor C Nnanatu

Open access · hybridAbstract readMeta-AnalysisSystematic Review
In one paragraph

Synthesis in Molecular ecology resources, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 2 pooled it
14.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 2 syntheses or guidelines pooled it, 95 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Philip D LambCentre for Environment, Fisheries and Aquaculture Science (Cefas), Lowestoft, Suffolk, UK.ORCID https://orcid.org/0000-0003-3748-8241
Vera G FonsecaCentre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth, Dorset, UK.ORCID https://orcid.org/0000-0003-1021-7003
David L MaxwellCentre for Environment, Fisheries and Aquaculture Science (Cefas), Lowestoft, Suffolk, UK.ORCID https://orcid.org/0000-0003-3943-0721
Centre for Environment, Fisheries and Aquaculture Science · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Environmental DNA (eDNA) has been used in a variety of ecological studies and management applications. The rate at which eDNA decays has been widely studied but at present it is difficult to disentangle study-specific effects from factors that universally affect eDNA degradation. To address this, a systematic review and meta-analysis was conducted on aquatic eDNA studies. Analysis revealed eDNA decayed faster at higher temperatures and in marine environments (as opposed to freshwater). DNA type (mitochondrial or nuclear) and fragment length did not affect eDNA decay rate, although a preference for <200 bp sequences in the available literature means this relationship was not assessed with longer sequences (e.g. >800 bp). At present, factors such as ultraviolet light, pH, and microbial load lacked sufficient studies to feature in the meta-analysis. Moving forward, we advocate researching these factors to further refine our understanding of eDNA decay in aquatic environments.

Indexed as

DNA, EnvironmentalDNAEnvironmental MonitoringFresh WaterTemperatureWaterDNADNA, EnvironmentalWateraquatic eDNAddPCReDNA concentrationeDNA degradationqPCRspecies detection

Identifiers

PMID35510730
PMCPMC9541873
OpenAlexW4229054411

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.