Evidence map›Paper›PMID 35510701›Full record

ArticleMolecular microbiology2022

BB0761, a MepM homolog, contributes to Borrelia burgdorferi cell division and mammalian infectivity.

Christina Thompson, Sierra George, Maria L White, Prahathees J Eswara, Zhiming Ouyang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Infection and immunity · 2025
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Christina ThompsonDepartment of Molecular Medicine, University of South Florida, Tampa, Florida, USA.
Sierra GeorgeDepartment of Molecular Medicine, University of South Florida, Tampa, Florida, USA.
Maria L WhiteDepartment of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, Florida, USA.
Prahathees J EswaraDepartment of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, Florida, USA.ORCID 0000-0003-4430-261X
Zhiming OuyangDepartment of Molecular Medicine, University of South Florida, Tampa, Florida, USA.ORCID 0000-0001-7427-1856
University of South Florida · US

Funding

Division site determination in Gram-positive bacteriaR35GM133617 · NIGMS · UNIVERSITY OF SOUTH FLORIDA · PI ESWARA, PRAHATHEES JAI · 2019 to 2023
$2.3M
Virulence regulation by BadR in the Lyme disease spirocheteR01AI152983 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI OUYANG, ZHIMING · 2021 to 2025
$1.9M
Analysis of the DksA regulon in the Lyme disease spirocheteR21AI159061 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI OUYANG, ZHIMING · 2021 to 2022
$411k
Virulence-associated genes in the Lyme disease spirocheteR03AI146909 · NIAID · UNIVERSITY OF SOUTH FLORIDA · PI OUYANG, ZHIMING · 2019 to 2020
$150k
NIAID NIH HHS R01 AI152983NIAID NIH HHS R03 AI146909NIAID NIH HHS R21 AI159061NIGMS NIH HHS R35 GM133617
6 · The paper itself

Abstract

M23 family endopeptidases play important roles in cell division and separation in a wide variety of bacteria. Recent studies have suggested that these proteins also contribute to bacterial virulence. However, the biological function of M23 peptidases in pathogenic spirochetes remains unexplored. Here, we describe Borrelia burgdorferi, the bacterial pathogen causing Lyme disease, requires a putative M23 family homolog, BB0761, for spirochete morphology and cell division. Indeed, the inactivation of bb0761 led to an aberrant filamentous phenotype as well as the impairment of B. burgdorferi growth in vitro. These phenotypes were complemented not only with B. burgdorferi bb0761, but also with the mepM gene from E. coli. Moreover, the bb0761 mutant showed a complete loss of infectivity in a murine model of Lyme borreliosis. Resistance of the mutant to osmotic and oxidative stresses was markedly reduced. Our combined results indicate that BB0761 contributes to B. burgdorferi cell division and virulence.

Indexed as

Borrelia burgdorferiLyme DiseaseAnimalsBacterial ProteinsCell DivisionEscherichia coliMammalsMiceBacterial ProteinsBorrelia burgdorferigene regulationlyme diseasepathogenesisproteasevirulence

Identifiers

PMID35510701
PMCPMC9794411
OpenAlexW4229035305

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.