Evidence map›Paper›PMID 35510260›Full record

ArticleData in brief2022

Proteomics dataset of epididymal fluid, seminal plasma, and proteins loosely attached to epididymal and ejaculated sperm from Angus bulls.

Saulo Menegatti Zoca, Emmalee J Northrop-Albrecht, Julie A Walker, Robert A Cushman, George A Perry

Open access · goldAbstract read
In one paragraph

Article in Data in brief, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Saulo Menegatti ZocaDepartment of Animal Science, South Dakota State University, Brookings, SD 57007, USA.
Emmalee J Northrop-AlbrechtDepartment of Animal Science, South Dakota State University, Brookings, SD 57007, USA.
Julie A WalkerDepartment of Animal Science, South Dakota State University, Brookings, SD 57007, USA.
Robert A CushmanUSDA, ARS, Roman L. Hruska US Meat Animal Research Center, Clay Center, NE 68933, USA.
George A PerryDepartment of Animal Science, South Dakota State University, Brookings, SD 57007, USA.
South Dakota State University · USRoman L. Hruska U.S. Meat Animal Research Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Peptides and proteins were identified by liquid chromatography with tandem mass spectrometry analysis (LCMS-MS) on an Orbitrap Velos mass spectrometer to further understand biological mechanisms that regulate increased longevity in epididymis compared to ejaculated sperm. Semen from sexually mature bulls were collected and then bulls were slaughtered to collect epididymal samples from the cauda epididymis. All samples were centrifuged to separate spermatozoa from fluids. A high ionic solution was used to remove surface proteins from spermatozoa. Four unique samples were generated: (1) epididymal fluid, (2) seminal plasma (ejaculated fluid), and proteins stripped from (3) epididymal sperm, and (4) ejaculated sperm. Samples were analyzed by LCMS-MS, and data were interpreted with Protein Pilot 5. False discovery rate (FDR) was set at 1%. Unique proteins (

Indexed as

BullEjaculationEpididymisProteinSpermatozoaSperm longevity

Identifiers

PMID35510260
PMCPMC9058950
OpenAlexW4224027323

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.