Evidence map›Paper›PMID 35509975›Full record

ArticleSTAR protocols2022

Analysis of brain region-specific mRNA synthesis and stability by utilizing adult mouse brain slice culture.

Volodymyr Dzhala, Alan J Fowler, Britt A DiMarzio, Kevin J Staley, Jaehong Suh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Volodymyr DzhalaDepartment of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA.
Alan J FowlerDepartment of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA.
Britt A DiMarzioDepartment of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA.
Kevin J StaleyDepartment of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA.
Jaehong SuhDepartment of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA.
Harvard University · USMaineGeneral Medical Center · USMassachusetts General Hospital · US

Funding

Role of Ataxin-1 in BACE1 Expression and Alzheimer's DiseaseR01AG056775 · NIA · MASSACHUSETTS GENERAL HOSPITAL · PI SUH, JAEHONG · 2018 to 2022
$2.1M
Timing of Neuronal Chloride Uptake for Control of Neonatal SeizureR01NS120973 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI Volodymyr Dzhala · 2022 to 2026
$2.0M
NIA NIH HHS R01 AG056775NINDS NIH HHS R01 NS120973
6 · The paper itself

Abstract

Utilization of live animals for mechanistic study is challenging yet pivotal to elucidate pathogenesis of neurological diseases. Here, we present a protocol that employs cultured brain slices derived from adult mice to examine mRNA metabolism. We describe the preparation of acute brain slices and the treatments of RNA synthesis inhibitor and nucleotide analog to examine the effects of ataxin-1 loss-of-function on

Indexed as

Amyloid Precursor Protein SecretasesAspartic Acid EndopeptidasesAnimalsBrainMiceNeuronsRNA, MessengerAmyloid Precursor Protein SecretasesAspartic Acid EndopeptidasesRNA, MessengerCell BiologyGene ExpressionGeneticsNeuroscience

Identifiers

PMID35509975
PMCPMC9059153
OpenAlexW4224304173

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.