Evidence map›Paper›PMID 35509970›Full record

ArticleSTAR protocols2022

High-yield isolation of pure fetal endothelial colony forming cells and mesenchymal stem cells from the human full-term placenta.

Rachel Nano, Seen Ling Sim, Abbas Shafiee, Kiarash Khosrotehrani, Jatin Patel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Rachel NanoCancer and Ageing Research Program, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Woolloongabba, QLD 4102, Australia.
Seen Ling SimThe University of Queensland Diamantina Institute, Faculty of Medicine, Woolloongabba, QLD 4102, Australia.
Abbas ShafieeHerston Biofabrication Institute, Metro North Hospital and Health Service, Brisbane, QLD 4029, Australia.
Kiarash KhosrotehraniThe University of Queensland Diamantina Institute, Faculty of Medicine, Woolloongabba, QLD 4102, Australia.
Jatin PatelCancer and Ageing Research Program, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Woolloongabba, QLD 4102, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A need to identify a stem cell source for human endothelial colony forming cells (ECFCs) and mesenchymal stem cells (MSCs) that is high yield is crucial for their implementation in ischemia. Our lab has developed an isolation protocol to do this using full-term human villous placental tissue. This protocol describes enzymatic tissue digestion followed by MACS and FACS, achieving an 8 times greater yield versus traditional isolation techniques and delivering pure fetal stem cell colonies within 21-28 days cell culture. For complete details on the use and execution of this protocol, please refer to Patel et al. (2013) and Patel et al. (2014).

Indexed as

Mesenchymal Stem CellsPlacentaCell Culture TechniquesEndothelial CellsFemaleFetusHumansPregnancyCell BiologyCell cultureCell isolationCell separation/fractionationFlow Cytometry/Mass CytometrySingle CellStem Cells

Identifiers

PMID35509970
PMCPMC9059155

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.