ArticleFrontiers in microbiology2022
It Takes Two to Tango: Combining Conventional Culture With Molecular Diagnostics Enhances Accuracy of
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Natural Carriage of Streptococcus pneumoniae Is Associated With Increased Experimental Pneumococcal Carriage but Reduced Conjugate Vaccine Efficacy in a Human Challenge Model.The Journal of infectious diseases · 2025Trial
- Diagnosis of pneumococcal pneumonia using quantitative PCR targeting lytA and piaB in adult patients with community-acquired pneumonia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Prevalence, Serotype Distribution, and Risk Factors Associated WithOpen forum infectious diseases · 2026Article
- Digital PCR linkage analysis resolvesMicrobiology spectrum · 2026Article
- Observational
- Detection of Pneumococcal Carriage in Asymptomatic Healthcare Workers.Open forum infectious diseases · 2025Article
- Clinical and Microbial Determinants of Upper Respiratory Colonization With Streptococcus pneumoniae and Native Microbiota in People With Human Immunodeficiency Virus Type 1 and Control Adults.The Journal of infectious diseases · 2024Article
- A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva.Microbiology spectrum · 2024Article
- Pneumococcal carriage in a large Sicilian sample population: impact on the current epidemiological scenario and implications for future vaccination strategies.Frontiers in cellular and infection microbiology · 2024Observational
- Impact of age on pneumococcal colonization of the nasopharynx and oral cavity: an ecological perspective.ISME communications · 2024Article
- Decreased Pneumococcal Carriage Among Older Adults in Denmark During the COVID-19 Lockdown.Open forum infectious diseases · 2023Article
- High Levels of Detection of Nonpneumococcal Species of Streptococcus in Saliva from Adults in the United States.Microbiology spectrum · 2023Article
- Article
- A spitting image: molecular diagnostics applied to saliva enhance detection ofFrontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
17 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: The specificity of molecular methods for the detection of Methods: Culture and qPCR methods were applied to detect pneumococcus and pneumococcal serotypes in 1,549 nasopharyngeal samples collected in the Netherlands ( Results: Detection of pneumococcus and pneumococcal serotypes with qPCRs in cultured (culture-enriched) nasopharyngeal samples exhibited near-perfect agreement with conventional culture (Cohen's kappa: 0.95). Molecular methods displayed increased sensitivity of detection for multiple serotype carriage, and implementation of qPCR-guided culturing significantly increased the proportion of nasopharyngeal and oropharyngeal samples from which live pneumococcus was recovered ( Conclusion: The accuracy of pneumococcal carriage surveillance can be greatly improved by complementing conventional culture with qPCR and
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.