Generation of Stable Catharanthus roseus Hairy Root Lines with Agrobacterium rhizogenes.

Krystyna K F Traverse, Samuel Mortensen, Juliet G Trautman, Hope Danison, Noreen F Rizvi, Carolyn W T Lee-Parsons

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

2.5field-weighted citation impact, top 10% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Krystyna K F Traverse *Department of Chemical Engineering, Northeastern University, Boston, MA, USA.

Samuel Mortensen *Department of Biology, Northeastern University, Boston, MA, USA.

Juliet G TrautmanDepartment of Bioengineering, Northeastern University, Boston, MA, USA.

Hope DanisonDepartment of Biology, Northeastern University, Boston, MA, USA.

Noreen F RizviDepartment of Chemical Engineering, Northeastern University, Boston, MA, USA.

Carolyn W T Lee-ParsonsDepartment of Chemical Engineering, Northeastern University, Boston, MA, USA. ca.lee@northeastern.edu.

Northeastern University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Agrobacterium rhizogenes is the bacterial agent that causes hairy root disease in dicots and is purposefully engineered for the development of transgenic hairy root cultures. Due to their genetic and metabolic stability, hairy root cultures offer advantages as a tissue culture system for investigating the function of transgenes and as a production platform for specialized metabolites or proteins. The process for generating hairy root cultures involves first infecting the explant with A. rhizogenes, excising and eliminating A. rhizogenes from the emerging hairy roots, selecting for transgenic hairy roots on plates containing the selective agent, confirming genomic integration of transgenes by PCR, and finally adapting the hairy roots in liquid media. Here we provide a detailed protocol for developing and maintaining transgenic hairy root cultures of our medicinal plant of interest, Catharanthus roseus.

Indexed as

CatharanthusAgrobacteriumPlant RootsPlants, Genetically ModifiedTransformation, GeneticAgrobacterium rhizogenesBiotechnologyCatharanthus roseusHairy rootsMetabolic engineeringPlant tissue cultureRhizobium rhizogenes

Identifiers

PMID35508835

DOI10.1104/pp.111.181834

OpenAlexW4285243781

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.