Evidence map›Paper›PMID 35508034›Full record

ArticleBrazilian dental journal

The response of Mesenchymal Stem Cells to endodontic materials.

Patrícia Yanne de Oliveira, Mariane Floriano Lopes Santos Lacerda, Carlos Magno da Costa Maranduba, João Vitor Paes Rettore, Leda Quercia Vieira, Antônio Paulino Ribeiro Sobrinho

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian dental journal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Patrícia Yanne de OliveiraDepartment of Operative Denstistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID http://orcid.org/0000-0003-0362-1040
Mariane Floriano Lopes Santos LacerdaDepartment of Operative Denstistry, School of Dentistry, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.ORCID http://orcid.org/0000-0002-1534-0150
Carlos Magno da Costa MarandubaDepartment of Genetics, Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.ORCID http://orcid.org/0000-0001-7327-1934
João Vitor Paes RettoreDepartment of Genetics, Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.ORCID http://orcid.org/0000-0002-8099-5145
Leda Quercia VieiraDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID http://orcid.org/0000-0001-5481-9618
Antônio Paulino Ribeiro SobrinhoDepartment of Operative Denstistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.ORCID http://orcid.org/0000-0002-3598-7592
Universidade Federal de Juiz de Fora · BRUniversidade Federal de Minas Gerais · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An endodontic material must be minimally harmful to stem cells since they are essential, thanks to their capacity for cell proliferation, self-renewal, and differentiation. For this reason, in this in vitro study, the cell viability and the expression of genes involved in cell plasticity and differentiation were investigated in stem cells recovered from human dental pulp (hDPSCs) that were in contact with four endodontic materials (Endofill, MTA, Pulp Canal Sealer, and Sealer 26). The viability of HDPSCs was assessed by MTT and trypan blue exclusion assays. PCR evaluated cellular plasticity by determining the CD34, CD45, Nestin, CD105, Nanog, and OCT4 expressions. The effect on cell differentiation was determined by RT-PCR expression of the RUNX2, ALP, OC/BGLAP, and DMP1 genes. The data were analyzed using ANOVA with Bonferroni correction (p <0.05). Pulp Canal Sealer and Endofill decreased cell viability after 48 hours (p <0.001). MTA and Sealer 26 did not disrupt cell viability (p> 0.05). When cultivated in the presence of MTA and Sealer 26, hDPSCs expressed Nestin, CD105, NANOG, and OCT-4 and did not express CD34 and CD45. MTA and Sealer 26 interfered with DMP1, OC/BGLAP and RUNX2 expressions (p <0.05) but did not change ALP gene expression (p> 0.05). MTA and Sealer 26 showed biological compatibility in the presence of hDPSCs.

Indexed as

Mesenchymal Stem CellsRoot Canal Filling MaterialsCalcium CompoundsCell DifferentiationCells, CulturedCore Binding Factor Alpha 1 SubunitDental PulpHumansNestinSilicatesCalcium CompoundsCore Binding Factor Alpha 1 SubunitNestinRoot Canal Filling MaterialsSilicates

Identifiers

PMID35508034
PMCPMC9645149
OpenAlexW4225117305

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.