Evidence map›Paper›PMID 35507985›Full record

ArticleJournal of applied oral science : revista FOB2022

Expression of anti-fungal peptide, β-defensin 118 in oral fibroblasts induced by C. albicans β-glucan-containing particles.

Miyuki Sakuma, Kouji Ohta, Shohei Fukada, Hiroki Kato, Takako Naruse, Takayuki Nakagawa, Hideo Shigeishi, Hiromi Nishi, Masaaki Takechi

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of applied oral science : revista FOB, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Miyuki SakumaHiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
Kouji OhtaHiroshima University, Graduate School of Biomedical and Health Sciences, Program of Oral Health Sciences, Department of Public Oral Health, Hiroshima, Japan.ORCID http://orcid.org/0000-0002-0603-9171
Shohei FukadaHiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
Hiroki KatoHiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
Takako NaruseHiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
Takayuki NakagawaHiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
Hideo ShigeishiHiroshima University, Graduate School of Biomedical and Health Sciences, Program of Oral Health Sciences, Department of Public Oral Health, Hiroshima, Japan.ORCID http://orcid.org/0000-0003-0883-4299
Hiromi NishiHiroshima University Hospital, Department of General Dentistry, Hiroshima, Japan.
Masaaki TakechiHiroshima University, Graduate School of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
Hiroshima University of Economics · JPHiroshima University Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveAlthough oral fibroblasts are thought to have the potential to enhance host defenses against Candida albicans , it is unknown whether they are able to recognize Candida cell components to increase the expression of antifungal peptides, such as defensin factors, against Candida infection. METHODOLOGY: We performed expression profiles of defensin genes induced by heat-killed C. albicans in oral immortalized fibroblasts (GT1) using cDNA microarray analysis. From those results, quantitative RT-PCR was used to examine the effects of Candida β-glucan-containing particles (β-GPs) on β-Defensin 118 (DEFB 118) expression in oral mucosal cells. Furthermore, the antifungal activities of recombinant DEFB 118 against C. albicans and C. glabrata were investigated using fungicidal assays.

resultsMicroarray analysis showed that DEFB118, β-Defensin 129 (DEFB129), and α-Defensin 1 (DEFA1) genes were induced by heat-killed C. albicans and that their mRNA expressions were also significantly increased by live as well as heat-killed C. albicans . Next, we focused on DEFB118, and found that GT1, primary fibroblasts, and RT7 (oral immortalized keratinocytes) constitutively expressed DEFB118 mRNA expression in RT-PCR. Furthermore, C. albicans β-GPs significantly increased the expression of DEFB118 mRNA in GT1 and primary fibroblasts. Although DEFB118 mRNA expression in RT7 was significantly induced by both live and heat-killed C. albicans, C. albicans β-GPs failed to have an effect on that expression. Finally, recombinant DEFB118 significantly decreased the survival of both strains of C. albicans in a dose-dependent manner, whereas no effects were seen for both C. glabrata strains.

conclusionDEFB118, induced by C. albicans β-GPs from oral fibroblasts, may play an important role in oral immune responses against C. albicans infection.

Indexed as

beta-Defensinsbeta-GlucansAntifungal AgentsCandida albicansFibroblastsGlucansRNA, MessengerAntifungal Agentsbeta-Defensinsbeta-GlucansGlucansRNA, Messenger

Identifiers

PMID35507985
PMCPMC9064192
OpenAlexW4226523924

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.