Application of 3D Culture Assays to Study Breast Morphogenesis, Epithelial Plasticity, and Cellular Interactions in an Epithelial Progenitor Cell Line.

Anna Karen Sigurdardottir, Bylgja Hilmarsdottir, Thorarinn Gudjonsson, Gunnhildur Asta Traustadottir

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Breast Morphogenesis: From Normal Development to Cancer.Advances in experimental medicine and biology · 2025
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Anna Karen SigurdardottirStem Cell Research Unit, Department of Anatomy, Faculty of Medicine, Biomedical Center, School of Health Sciences, University of Iceland, Reykjavik, Iceland.

Bylgja HilmarsdottirDepartment of Pathology, Landspitali-University Hospital, Reykjavik, Iceland.ORCID 0000-0001-8693-5541

Thorarinn GudjonssonStem Cell Research Unit, Department of Anatomy, Faculty of Medicine, Biomedical Center, School of Health Sciences, University of Iceland, Reykjavik, Iceland.ORCID 0000-0001-9645-9665

Gunnhildur Asta TraustadottirStem Cell Research Unit, Department of Anatomy, Faculty of Medicine, Biomedical Center, School of Health Sciences, University of Iceland, Reykjavik, Iceland. gutra@hi.is.ORCID 0000-0001-6044-0989

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Capturing breast morphogenesis and cancer progression in 3D culture using cell lines with stem cell properties can greatly increase understanding of the underlying mechanisms involved in these processes, highlighting the importance of the culture method. D492 is a breast epithelial progenitor cell line that provides a model for branching morphogenesis when cultured in 3D reconstituted basement membrane matrix (rBM). Along with its derivate cell lines D492M and D492HER2, D492 also serves as a robust model for epithelial to mesenchymal transition (EMT) and tumorigenicity, respectively. Here, we describe the routine maintenance and application of the D492 cell lines in 3D culture for the study of branching morphogenesis, EMT and epithelial-endothelial interaction.

Indexed as

Epithelial CellsEpithelial-Mesenchymal TransitionCell CommunicationMorphogenesisStem Cells3D cultureBranching morphogenesisBreast endothelial cells (BRENCs)D492 cell linesMatrigel®

Identifiers

PMID35507176

DOI10.1038/nmeth1015

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.