Evidence map›Paper›PMID 35507158›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Assessment of Endothelial-to-Hematopoietic Transition of Individual Hemogenic Endothelium and Bulk Populations in Defined Conditions.

Gene I Uenishi, Ho Sun Jung, Igor I Slukvin

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 74% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Gene I UenishiWisconsin National Primate Research Center, University of Wisconsin Graduate School, Madison, WI, USA.
Ho Sun JungDepartment of Cell and Regenerative Biology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
Igor I SlukvinWisconsin National Primate Research Center, University of Wisconsin Graduate School, Madison, WI, USA. islukvin@wisc.edu.
University of Wisconsin–Madison · US

Funding

WNPRC Supplemental Request for Nonhuman Primate Enclosures to Equip HIV/AIDS-Related Research FacilitiesP51OD011106 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI Dorota A. Grejner-Brzezinska · 2012 to 2026
$150.7M
Transplantation of MHC Homozygous Vascular Progenitors in PrimatesU01HL134655 · NHLBI · MORGRIDGE INSTITUTE FOR RESEARCH, INC. · PI SLUKVIN, IGOR I. · 2016 to 2022
$7.7M
Molecular Determinants of Hemogenic EndotheliumR01HL142665 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI SLUKVIN, IGOR I. · 2018 to 2021
$2.0M
NHLBI NIH HHS R01 HL142665NHLBI NIH HHS U01 HL134655NIH HHS P51 OD011106
6 · The paper itself

Abstract

Endothelial-to-hematopoietic transition (EHT) is a unique morphogenic event in which flat, adherent hemogenic endothelial (HE) cells acquire round, non-adherent blood cell morphology. Investigating the mechanisms of EHT is critical for understanding the development of hematopoietic stem cells (HSCs) and the entirety of the adult immune system, and advancing technologies for manufacturing blood cells from human pluripotent stem cells (hPSCs). Here we describe a protocol to (a) generate and isolate subsets of HE from hPSCs, (b) assess EHT and hematopoietic potential of HE subsets in bulk cultures and at the single-cell level, and (c) evaluate the role of NOTCH signaling during HE specification and EHT. The generation of HE from hPSCs and EHT bulk cultures are performed in xenogen- and feeder-free system, providing the unique advantage of being able to investigate the role of individual signaling factors during EHT and the definitive lympho-myeloid cell specification from hPSCs.

Indexed as

HemangioblastsPluripotent Stem CellsCell DifferentiationHematopoiesisHematopoietic Stem CellsHumansEndothelial-to-hematopoietic transitionHematopoiesisHemogenic endotheliumHuman pluripotent stem cellsNotch signaling

Identifiers

PMID35507158
OpenAlexW4285181117

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.