Evidence map›Paper›PMID 35507157›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Extracellular Flux Analysis of Mitochondrial Function in Pluripotent Stem Cells.

Enkhtuul Tsogtbaatar, Katherine Minter-Dykhouse, Alicia Saarinen, Clifford D L Folmes

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Enkhtuul TsogtbaatarStem Cell and Regenerative Metabolism Laboratory, Departments of Cardiovascular Medicine, Biochemistry and Molecular Biology, and Center for Regenerative Medicine, Mayo Clinic, Scottsdale, AZ, USA.
Katherine Minter-DykhouseStem Cell and Regenerative Metabolism Laboratory, Departments of Cardiovascular Medicine, Biochemistry and Molecular Biology, and Center for Regenerative Medicine, Mayo Clinic, Scottsdale, AZ, USA.
Alicia SaarinenStem Cell and Regenerative Metabolism Laboratory, Departments of Cardiovascular Medicine, Biochemistry and Molecular Biology, and Center for Regenerative Medicine, Mayo Clinic, Scottsdale, AZ, USA.
Clifford D L FolmesStem Cell and Regenerative Metabolism Laboratory, Departments of Cardiovascular Medicine, Biochemistry and Molecular Biology, and Center for Regenerative Medicine, Mayo Clinic, Scottsdale, AZ, USA. folmes.clifford@mayo.edu.
Mayo Clinic in Arizona · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mitochondrial function and energy metabolism are increasingly recognized not only as regulators of pluripotent stem cell function and fate, but also as critical targets in disease pathogenesis and aging. Therefore across the downstream applications of pluripotent stem cells, including development and disease modeling, drug screening, and cell-based therapies, it is crucial to be able to measure mitochondrial function and metabolism in a high-throughput, real-time and label-free manner. Here we describe the application of Seahorse extracellular flux analysis to measure mitochondrial function in pluripotent stem cells and their derivatives. Specifically, we highlight two assays, the Mitochondrial Stress Test, which quantifies overall mitochondrial function including basal, maximal and ATP-couple oxygen consumption rates, and the Electron Transport Chain Complex Specific assay, that quantifies function of individual complexes within the electron transport chain.

Indexed as

Pluripotent Stem CellsEnergy MetabolismMitochondriaOxygen ConsumptionDifferentiationEmbryonic stem cellsInduced pluripotent stem cellsMitochondrial respirationOxidative metabolismOxidative phosphorylation

Identifiers

PMID35507157
OpenAlexW4285284911

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.