Evidence map›Paper›PMID 35507153›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Generation of Human iPSC from Small Volume Peripheral Blood Samples.

Doreen Kloos, Nico Lachmann

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Doreen KloosInstitute of Experimental Hematology, Hannover Medical School, Hannover, Germany.
Nico LachmannREBIRTH, Research Center for Translational and Regenerative Medicine, Hannover Medical School, Hannover, Germany. Lachmann.nico@mh-hannover.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The generation of induced pluripotent stem cells (iPSCs) from patients has opened new doors to gain insights into disease pathophysiology and treatment. In particular, the generation of iPSCs from patients who suffer from inherited diseases is of great interest, as most of these diseases are rare and not well studied. As most affected patients are diagnosed during infancy, the derivation of somatic cells for the generation of iPSCs is very much limited. Here we describe a protocol for the generation of human iPSCs from non-mobilized peripheral blood. This protocol can be adapted to all volumes of blood, starting from 1 ml of peripheral blood. Isolated and processed cells can be used for the generation of iPSCs by both, lentiviral and Sendai virus mediated reprogramming, allowing for the rapid generation of patient-specific iPSCs.

Indexed as

Induced Pluripotent Stem CellsCell DifferentiationCellular ReprogrammingGenetic VectorsHumansLeukocytes, MononuclearSendai virusiPSCLentivirusPeripheral bloodReprogramming

Identifiers

PMID35507153

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.