Evidence map›Paper›PMID 35507110›Full record

ArticleMikrochimica acta2022

Internal heating method of loop-mediated isothermal amplification for detection of HPV-6 DNA.

Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu

Abstract read
In one paragraph

Article in Mikrochimica acta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Huan-Huan ZhuDepartment of Central Laboratory, Clinical Medicine Scientific and Technical Innovation Park, Shanghai Tenth People's Hospital, Shanghai, 200435, China.
Yuan LiDepartment of Clinical Laboratory, Yangpu Hospital, Tongji University School of Medicine, Shanghai, 200090, China.
Li-Xia WuDepartment of Clinical Laboratory Medicine, Children's Hospital of Soochow University, Medical College of Soochow University, Suchow, 215025, China.
Ke-Sheng WangDepartment of Central Laboratory, Clinical Medicine Scientific and Technical Innovation Park, Shanghai Tenth People's Hospital, Shanghai, 200435, China.
Yue ZhangDepartment of Central Laboratory, Clinical Medicine Scientific and Technical Innovation Park, Shanghai Tenth People's Hospital, Shanghai, 200435, China.
Qiang-Yuan FanDepartment of Central Laboratory, Clinical Medicine Scientific and Technical Innovation Park, Shanghai Tenth People's Hospital, Shanghai, 200435, China.
Zun-Zhen MingDepartment of Central Laboratory, Clinical Medicine Scientific and Technical Innovation Park, Shanghai Tenth People's Hospital, Shanghai, 200435, China.
Wei-Qin ChenDepartment of Laboratory Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. wonderforjune@126.com.
Wei-Wei LiuDepartment of Central Laboratory, Clinical Medicine Scientific and Technical Innovation Park, Shanghai Tenth People's Hospital, Shanghai, 200435, China. huashanvivian@126.com.

Funding

Special Clinical Research Project of Shanghai Municipal Health Commission 202140147the Outstanding Academic Leaders Plan of Shanghai 2018BR07
6 · The paper itself

Abstract

Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, which is known for its rapid process, simple operation, high amplification efficiency, and excellent sensitivity. However, most of the existing heating methods are external for completion of molecular amplification with possibility of contamination of specimens. The present research provided an internal heating method for LAMP using magnetic nanoparticles (MNPs), which is called nano-LAMP. Near-infrared light with an excitation wavelength of 808 nm was employed as the heating source; hydroxy naphthol blue (HNB) was used as an indicator to conduct methodological research. We demonstrate that the best temperature was controlled at a working power of 2 W and 4.8 µg/µL concentration of nanoparticles. The lowest limit for the detection of HPV by the nano-LAMP method is 10

Indexed as

Nucleic Acid Amplification TechniquesDNAHot TemperatureHuman papillomavirus 6Molecular Diagnostic TechniquesSensitivity and SpecificityDNAGenetic amplificationHuman papillomavirusLoop-mediated isothermal amplificationMagnetic nanoparticlesNano-LAMP

Identifiers

PMID35507110
PMCPMC9065241

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.