ArticleACS central science2022
Minimalist Design of an Intrinsically Disordered Protein-Mimicking Scaffold for an Artificial Membraneless Organelle.
Article in ACS central science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 90 citations in OpenAlex.
- Intrinsic Disorder as a Biomimetic Design Paradigm.Biomimetics (Basel, Switzerland) · 2026Article
- Construction of artificial organellesChemical science · 2025Review
- Article
- A designer minimalistic model parallels the phase-separation-mediated assembly and biophysical cues of extracellular matrix.Nature chemistry · 2025Article
- Time-dependent catalytic activity in aging condensates.Nature communications · 2025Article
- Phase Separation Clustering of Poly Ubiquitin Cargos on Ternary Mixture Lipid Membranes by Synthetically Cross-Linked Ubiquitin Binder Peptides.Biochemistry · 2025Article
- Size-controlled assembly of phase separated protein condensates with interfacial protein cages.Nature communications · 2025Article
- Sequestration within peptide coacervates improves the fluorescence intensity, kinetics, and limits of detection of dye-based DNA biosensors.Communications chemistry · 2024Article
- Modulating hierarchical self-assembly in thermoresponsive intrinsically disordered proteins through high-temperature incubation time.Scientific reports · 2023Article
- Microscopic Droplet Size Analysis (MDSA) of "Five Thieves' Oil" (Olejek Pięciu Złodziei) Essential Oil after the Nebulization Process.Molecules (Basel, Switzerland) · 2023Article
- Review
- Biomimetic Liquid Metal-Elastomer Composited Foam with Adjustable Thermal Conductivity for Heat Control.Molecules (Basel, Switzerland) · 2023Article
- Construction of multiphasic membraneless organelles towards spontaneous spatial segregation and directional flow of biochemical reactions.Chemical science · 2023Article
- Recent Advances in Polymer Additive Engineering for Diagnostic and Therapeutic Hydrogels.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liquid-liquid phase separation (LLPS) is an emerging and universal mechanism for intracellular organization, particularly, by forming membraneless organelles (MLOs) hosting intrinsically disordered proteins (IDPs) as scaffolds. Genetic engineering is generally applied to reconstruct IDPs harboring over 100 amino acid residues. Here, we report the first design of synthetic hybrids consisting of short oligopeptides of fewer than 10 residues as "stickers" and dextran as a "spacer" to recapitulate the characteristics of IDPs, as exemplified by the multivalent FUS protein. Hybrids undergo LLPS into micron-sized liquid droplets resembling LLPS in vitro and in living cells. Moreover, the droplets formed are capable of recruiting proteins and RNAs and providing a favorable environment for a biochemical reaction with highly enriched components, thereby mimicking the function of natural MLOs. This simple yet versatile model system can help elucidate the molecular interactions implicated in MLOs and pave ways to a new type of biomimetic materials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.