ArticleThe EMBO journal2022
Centrioles generate a local pulse of Polo/PLK1 activity to initiate mitotic centrosome assembly.
Article in The EMBO journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Polo/PLK1 phosphorylation relieves Centrosomin/Cnn autoinhibition to promote centrosome scaffold assembly.The EMBO journal · 2026Article
- Bora, CEP192 and Cenexin regulate distinct Plk1-dependent cell and centrosome cycle transitions.Nature communications · 2026Article
- NuSAP Safeguards Centriole Integrity to Mediate CEP57-CEP152 Torus Recruitment for Proper Engagement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Radial Profile-Based Quantification of Centrosomal Proteins.Bio-protocol · 2026Article
- A cell cycle-dependent transition of acetylation to phosphorylation regulates timely centrosome maturation.Nature communications · 2026Article
- Inactivation of microtubule organizing center function of the centrosome is required for neuronal development and centriole elimination.bioRxiv : the preprint server for biology · 2025Article
- In vitro reconstitution of a minimal human centrosome scaffold capable of forming and clustering microtubule asters.Journal of cell science · 2025Article
- The conserved Spd-2/CEP192 domain adopts a unique protein fold to promote centrosome scaffold assembly.Science advances · 2025Article
- Article
- Centrioles generate two scaffolds with distinct biophysical properties to build mitotic centrosomes.Science advances · 2025Article
- Catalytic growth in a shared enzyme pool ensures robust control of centrosome size.bioRxiv : the preprint server for biology · 2024Article
- Regulation of centrosome size by the cell-cycle oscillator in Drosophila embryos.The EMBO journal · 2024Article
- Centrosomes and associated proteins in pathogenesis and treatment of breast cancer.Frontiers in oncology · 2024Review
- The centrosome - diverse functions in fertilization and development across species.Journal of cell science · 2023Review
- SH2D4A promotes centrosome maturation to support spindle microtubule formation and mitotic progression.Scientific reports · 2023Article
- Multiple Roles of PLK1 in Mitosis and Meiosis.Cells · 2023Review
- Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL.The Journal of cell biology · 2022Article
- Pericentriolar matrix (PCM) integrity relies on cenexin and polo-like kinase (PLK)1.Molecular biology of the cell · 2022Article
- Centriole distal-end proteins CP110 and Cep97 influence centriole cartwheel growth at the proximal end.Journal of cell science · 2022Article
- Centrioles generate a local pulse of Polo/PLK1 activity to initiate mitotic centrosome assembly.The EMBO journal · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Mitotic centrosomes are formed when centrioles start to recruit large amounts of pericentriolar material (PCM) around themselves in preparation for mitosis. This centrosome "maturation" requires the centrioles and also Polo/PLK1 protein kinase. The PCM comprises several hundred proteins and, in Drosophila, Polo cooperates with the conserved centrosome proteins Spd-2/CEP192 and Cnn/CDK5RAP2 to assemble a PCM scaffold around the mother centriole that then recruits other PCM client proteins. We show here that in Drosophila syncytial blastoderm embryos, centrosomal Polo levels rise and fall during the assembly process-peaking, and then starting to decline, even as levels of the PCM scaffold continue to rise and plateau. Experiments and mathematical modelling indicate that a centriolar pulse of Polo activity, potentially generated by the interaction between Polo and its centriole receptor Ana1 (CEP295 in humans), could explain these unexpected scaffold assembly dynamics. We propose that centrioles generate a local pulse of Polo activity prior to mitotic entry to initiate centrosome maturation, explaining why centrioles and Polo/PLK1 are normally essential for this process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.