Evidence map›Paper›PMID 35505044›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Optimal Use of 2',7'-Dichlorofluorescein Diacetate in Cultured Hepatocytes.

Megan J Reiniers, Lianne R de Haan, Laurens F Reeskamp, Mans Broekgaarden, Ruurdtje Hoekstra, Rowan F van Golen, Michal Heger

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
5.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 3 countries.

Megan J ReiniersJiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, College of Medicine, Jiaxing University, Jiaxing, Zhejiang, P. R. China.
Lianne R de HaanJiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, College of Medicine, Jiaxing University, Jiaxing, Zhejiang, P. R. China.
Laurens F ReeskampDepartment of Vascular Medicine, Amsterdam UMC, Location AMC, Amsterdam, The Netherlands.
Mans BroekgaardenTeam Cancer Targets and Experimental Therapeutics, Department Microenvironment Cell Plasticity and Signaling, Institute for Advanced Biosciences, Université de Grenoble-Alpes, Allée des Alpes, La Tronche, France.
Ruurdtje HoekstraTytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location AMC, Amsterdam, The Netherlands.
Rowan F van GolenDepartment of Gastroenterology and Hepatology, Leiden University Medical Center, Leiden, The Netherlands. R.F.van_Golen@lumc.nl.
Michal HegerJiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, College of Medicine, Jiaxing University, Jiaxing, Zhejiang, P. R. China. m.heger@uu.nl.
Amsterdam University Medical Centers · NLJiaxing University · CNErasmus MC · NLInserm · FRLeiden University Medical Center · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oxidative stress is a state that arises when the production of reactive transients overwhelms the cell's capacity to neutralize the oxidants and radicals. This state often coincides with the pathogenesis and perpetuation of numerous chronic diseases. On the other hand, medical interventions such as radiation therapy and photodynamic therapy generate radicals to selectively damage and kill diseased tissue. As a result, the qualification and quantification of oxidative stress are of great interest to those studying disease mechanisms as well as therapeutic interventions. 2',7'-Dichlorodihydrofluorescein-diacetate (DCFH

Indexed as

HepatocytesOxidative StressFluoresceinsHumansOxidation-ReductiondiacetyldichlorofluoresceinFluoresceins2′,7′-Dichlorodihydrofluorescein-diacetate2′,7′-DichlorofluoresceinCellular uptake and effluxFluorogenic redox probeHepatocytesOxidative and nitrosative stress

Identifiers

PMID35505044
OpenAlexW4285219526

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.