Evidence map›Paper›PMID 35501883›Full record

ArticleBiotechnology for biofuels and bioproducts2022

The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae.

Lin Shu, Jinjie Gu, Qinghui Wang, Shaoqi Sun, Youtian Cui, Jason Fell, Wai Shun Mak, Justin B Siegel, Jiping Shi, Gary J Lye and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology for biofuels and bioproducts, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Lin Shu *Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
Jinjie Gu *Lab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
Qinghui WangLab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
Shaoqi SunLab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
Youtian CuiDepartment of Chemistry, Biochemistry & Molecular Medicine, and the Genome Center, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.
Jason FellDepartment of Chemistry, Biochemistry & Molecular Medicine, and the Genome Center, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.
Wai Shun MakDepartment of Chemistry, Biochemistry & Molecular Medicine, and the Genome Center, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.
Justin B SiegelDepartment of Chemistry, Biochemistry & Molecular Medicine, and the Genome Center, University of California, Davis, One Shields Avenue, Davis, CA, 95616, USA.
Jiping ShiLab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China.
Gary J LyeDepartment of Biochemical Engineering, University College London, Gordon Street, London, WC1H 0AH, UK.
Frank BaganzDepartment of Biochemical Engineering, University College London, Gordon Street, London, WC1H 0AH, UK. f.baganz@ucl.ac.uk.
Jian HaoLab of Biorefinery, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 99 Haike Road, Pudong, Shanghai, 201210, People's Republic of China. haoj@sari.ac.cn.ORCID http://orcid.org/0000-0002-4403-8188
Chinese Academy of Sciences · CNUniversity of California, Davis · USUniversity College London · GBShanghai Advanced Research Institute · CN

Funding

Key Technologies Research and Development Program 2019YFE0196900Natural Science Foundation of Shanghai 19ZR1463600Royal Society-Newton Advanced Fellowship NAF\R2\180721
6 · The paper itself

Abstract

backgroundKlebsiella pneumoniae contains an endogenous isobutanol synthesis pathway. The ipdC gene annotated as an indole-3-pyruvate decarboxylase (Kp-IpdC), was identified to catalyze the formation of isobutyraldehyde from 2-ketoisovalerate.

resultsCompared with 2-ketoisovalerate decarboxylase from Lactococcus lactis (KivD), a decarboxylase commonly used in artificial isobutanol synthesis pathways, Kp-IpdC has an 2.8-fold lower K

conclusionsThis research provides a new way to improve the efficiency of the biological route of isobutanol production.

Indexed as

2-Ketoisovalerate decarboxylaseIndole-3-pyruvate decarboxylaseIsobutanolKlebsiella pneumoniae

Identifiers

PMID35501883
PMCPMC9063327
OpenAlexW4225278936

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.