ArticleNature methods2022
Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging.
Article in Nature methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
What it found
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Who cites it
63 citing papers in PubMed.
- Brightness demixing for simultaneous multi-target imaging in 3D single-molecule localization microscopy.Nature methods · 2026Article
- FINDER converts zero-background kinetic fingerprinting into area-scalable attomolar biomarker detection.Research square · 2026Article
- Mitotic Cdc42 waves encode PI(3,4)PScience advances · 2026Article
- FINDER converts zero-background kinetic fingerprinting into area-scalable attomolar biomarker detection.bioRxiv : the preprint server for biology · 2026Article
- Accelerated Left-Handed DNA-PAINT Using Fluorogenic Probes.Nano letters · 2026Article
- The molecular basis of force selectivity by PIEZO2.Nature · 2026Article
- BLeaching In-cell Single-molecule burstS (BLISS) reveals a small dynamic fraction of HP1α clusters in undifferentiated embryonic stem cells.bioRxiv : the preprint server for biology · 2026Article
- Mirror-enhanced 4Pi-SMLM with one objective enables isotropic nanoscale imaging.Nature biotechnology · 2026Article
- Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells.bioRxiv : the preprint server for biology · 2026Article
- Impact of Docking Strand Design on Spatial Resolution in DNA-Points Accumulation for Imaging in Nanoscale Topography.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- High-Throughput Hyperspectral and Multiplexed Super-Resolution Fluorescence Imaging by SP-STORM.JACS Au · 2026Article
- A Simple, Ultrastable, and Cost-Effective Oxygen-Scavenging System for Long-Term DNA-PAINT Imaging.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Versatile introduction of multifunctional Michael-acceptor moieties on amino-oligonucleotides for bioconjugation purposes.Communications chemistry · 2026Article
- Optimizing Effective Labeling Efficiency in MINFLUX 3D DNA-PAINT Microscopy by Maximizing Marker Detection Probability.ACS photonics · 2026Article
- Stemless molecular beacon probes for single-molecule detection of supercoil-induced DNA denaturation.npj biological physics and mechanics · 2026Article
- 4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination.Nature methods · 2026Article
- Evaluating MINFLUX experimental performance in silico.Nature communications · 2025Article
- FRETsael: Nanometer localization of biomolecular interactions using fluorescence lifetime imaging.Biophysical journal · 2025Article
- A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context.bioRxiv : the preprint server for biology · 2025Article
- Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope.Nature communications · 2025Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
DNA-based points accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution microscopy method that can acquire high-fidelity images at nanometer resolution. It suffers, however, from high background and slow imaging speed, both of which can be attributed to the presence of unbound fluorophores in solution. Here we present two-color fluorogenic DNA-PAINT, which uses improved imager probe and docking strand designs to solve these problems. These self-quenching single-stranded DNA probes are conjugated with a fluorophore and quencher at the terminals, which permits an increase in fluorescence by up to 57-fold upon binding and unquenching. In addition, the engineering of base pair mismatches between the fluorogenic imager probes and docking strands allowed us to achieve both high fluorogenicity and the fast binding kinetics required for fast imaging. We demonstrate a 26-fold increase in imaging speed over regular DNA-PAINT and show that our new implementation enables three-dimensional super-resolution DNA-PAINT imaging without optical sectioning.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.