ArticlePLoS pathogens2022
Vibrio cholerae requires oxidative respiration through the bd-I and cbb3 oxidases for intestinal proliferation.
Article in PLoS pathogens, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- The role of cytochrome oxidases in bacterial virulence.Infection and immunity · 2026Review
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- A cytochromeApplied and environmental microbiology · 2025Article
- Understanding bovine genital campylobacteriosis: A comprehensive review of its threat to global cattle reproduction.Open veterinary journal · 2025Review
- NQR as a target for new antibiotics.Frontiers in microbiology · 2025Review
- Carbapenem-ResistantbioRxiv : the preprint server for biology · 2024Article
- A long journey to the colon: The role of the small intestine microbiota in intestinal disease.Molecular microbiology · 2024Review
- Cytochrome oxidase requirements in Bordetella reveal insights into evolution towards life in the mammalian respiratory tract.PLoS pathogens · 2024Article
- Gut metabolite L-lactate supportsProceedings of the National Academy of Sciences of the United States of America · 2024Article
- DegS regulates the aerobic metabolism ofFrontiers in cellular and infection microbiology · 2024Article
- Modulation of Host-Microbe Metabolism by Cholera Toxin.Infection and immunity · 2023Review
- Genome-wide association studies reveal distinct genetic correlates and increased heritability of antimicrobial resistance inMicrobial genomics · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio cholerae respires both aerobically and anaerobically and, while oxygen may be available to it during infection, other terminal electron acceptors are proposed for population expansion during infection. Unlike gastrointestinal pathogens that stimulate significant inflammation leading to elevated levels of oxygen or alternative terminal electron acceptors, V. cholerae infections are not understood to induce a notable inflammatory response. To ascertain the respiration requirements of V. cholerae during infection, we used Multiplex Genome Editing by Natural Transformation (MuGENT) to create V. cholerae strains lacking aerobic or anaerobic respiration. V. cholerae strains lacking aerobic respiration were attenuated in infant mice 105-fold relative to wild type, while strains lacking anaerobic respiration had no colonization defect, contrary to earlier work suggesting a role for anaerobic respiration during infection. Using several approaches, including one we developed for this work termed Comparative Multiplex PCR Amplicon Sequencing (CoMPAS), we determined that the bd-I and cbb3 oxidases are essential for small intestinal colonization of V. cholerae in the infant mouse. The bd-I oxidase was also determined as the primary oxidase during growth outside the host, making V. cholerae the only example of a Gram-negative bacterial pathogen in which a bd-type oxidase is the primary oxidase for energy acquisition inside and outside of a host.
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