Evidence map›Paper›PMID 35496800›Full record

ArticleSTAR protocols2022

Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup.

Jonas Hyld Steffen, Julie Winkel Missel, Tamim Al-Jubair, Philip Kitchen, Mootaz M Salman, Roslyn M Bill, Susanna Törnroth-Horsefield, Pontus Gourdon

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Jonas Hyld SteffenDepartment of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Julie Winkel MisselDepartment of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Tamim Al-JubairDepartment of Biochemistry and Structural Biology, Lund University, P.O. Box 124, 221 00 Lund, Sweden.
Philip KitchenCollege of Health and Life Sciences, Aston University, Aston Triangle, B4 7ET Birmingham, UK.
Mootaz M SalmanDepartment of Physiology, Anatomy and Genetics, Kavli Institute for NanoScience Discovery, University of Oxford, Parks Road, OX1 3PT Oxford, UK.
Roslyn M BillCollege of Health and Life Sciences, Aston University, Aston Triangle, B4 7ET Birmingham, UK.
Susanna Törnroth-HorsefieldDepartment of Biochemistry and Structural Biology, Lund University, P.O. Box 124, 221 00 Lund, Sweden.
Pontus GourdonDepartment of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.

Funding

Biotechnology and Biological Sciences Research Council BB/P025927/1
6 · The paper itself

Abstract

Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes. Using a stopped-flow setup, AQP embedded in proteoliposomes are exposed to an osmogenic gradient that triggers water flux. The consequent effects on proteoliposome size can be tracked using the fluorescence of an internalized fluorophore. This enables controlled characterization of water flux by AQPs. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020).

Indexed as

AquaporinsWaterPermeabilityProteolipidsAquaporinsProteolipidsproteoliposomesWaterCell MembraneProtein BiochemistrySingle-molecule Assays

Identifiers

PMID35496800
PMCPMC9038760

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.