Evidence map›Paper›PMID 35496798›Full record

ArticleSTAR protocols2022

Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast.

Andrew K Lamb, Santiago M Di Pietro

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 61% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Andrew K LambDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870, USA.
Santiago M Di PietroDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870, USA.
Colorado State University · US

Funding

Molecular Mechanisms of Pigmentation in Health and DiseaseR01GM125619 · NIGMS · COLORADO STATE UNIVERSITY · PI DI PIETRO, SANTIAGO MAURO · 2018 to 2021
$1.4M
NIGMS NIH HHS R01 GM125619
6 · The paper itself

Abstract

Chemically induced dimerization (CID) is a useful tool for artificially inducing protein-protein interactions. Although CID has been used extensively for live-cell microscopy applications in mammalian systems, it is rarely utilized in yeast cell biology studies. Here, we present a step-by-step protocol for the utilization of a CID system in live-cell microscopy experiments of budding yeast endocytosis. While focusing on the study of endocytosis, this protocol framework is adaptable to the study of other cellular processes in

Indexed as

SaccharomycetalesAnimalsDimerizationEndocytosisMammalsSaccharomyces cerevisiaeSheepTacrolimus Binding ProteinsTacrolimus Binding ProteinsCell-based AssaysCell BiologyMicroscopyModel OrganismsSignal Transduction

Identifiers

PMID35496798
PMCPMC9038778
OpenAlexW4224088359

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.