Evidence map›Paper›PMID 35496778›Full record

ArticleSTAR protocols2022

Transmission electron microscopic analysis of myelination in the murine central nervous system.

Yan Wang, Bo Sun, Bradley Shibata, Fuzheng Guo

Abstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yan WangDepartment of Neurology, School of Medicine, University of California, Davis, Sacramento, CA 95817, USA.
Bo SunInstitute for Pediatric Regenerative Medicine (IPRM), Shriners Hospitals for Children - Northern California, Sacramento, CA 95817, USA.
Bradley ShibataBiological Electron Microscopy Facility, University of California, Davis, CA 95616, USA.
Fuzheng GuoDepartment of Neurology, School of Medicine, University of California, Davis, Sacramento, CA 95817, USA.

Funding

The curious case of PARP1 in CNS myelin formation and repairR01NS123165 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Fuzheng Guo · 2022 to 2026
$2.5M
Glial HIFa: mechanisms and implications in hypoxia/ischemia-induced oligodendroglial pathologyR01NS123080 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI GUO, FUZHENG · 2021 to 2025
$2.3M
The Wnt-independent role of TCF7l2 in CNS myelin formation and repairR01NS094559 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI GUO, FUZHENG · 2016 to 2020
$1.4M
Using genetic approaches to explore the role of group I PAKs in developmental myelination of the mammalian CNSR21NS125464 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI GUO, FUZHENG · 2022 to 2022
$439k
A paradigm shift in our understanding of oligodendroglial HIFa-regulated CNS angiogenesisR21NS109790 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI GUO, FUZHENG · 2018 to 2019
$432k
NINDS NIH HHS R01 NS094559NINDS NIH HHS R01 NS123080NINDS NIH HHS R01 NS123165NINDS NIH HHS R21 NS109790NINDS NIH HHS R21 NS125464
6 · The paper itself

Abstract

Myelin provides physical, neurotrophic, and metabolic support for axonal integrity. The thickness of CNS (central nervous system) myelin sheath is usually < one micrometer, which is under or near the detection threshold of the conventional light microscopy. Here, we present a high-resolution transmission electron microscopy-based protocol to assess myelination at the ultrastructural level. We describe sample preparation from mouse tissue, followed by electron microscopic imaging and CNS myelination analysis. This protocol is also useful for analyzing murine PNS myelination. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).

Indexed as

Central Nervous SystemElectronsAnimalsAxonsMiceMicroscopy, Electron, TransmissionMyelin SheathDevelopmental biologyMicroscopyModel OrganismsNeuroscience

Identifiers

PMID35496778
PMCPMC9043565

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.