ArticleRSC advances2020
Microfluidic paper device for rapid detection of aflatoxin B1 using an aptamer based colorimetric assay.
Article in RSC advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Aflatoxin exposure and health impacts: global burden and advances in detection technologies.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2026Review
- Aptamer discovery via GO-SELEX and GrNP-SELEX for monocrotophos and its comparative analysis.Mikrochimica acta · 2026Article
- Emerging biosensors integrated with microfluidic devices: a promising analytical tool for on-site detection of mycotoxins.NPJ science of food · 2025Review
- Assembly of adaptive engineering aptamers for Escherichia coli and their application in all-in-one rapid detection.Mikrochimica acta · 2025Article
- Microfluidic paper analytic device (μPAD) technology for food safety applications.Biomicrofluidics · 2024Review
- Emerging 0D, 1D, 2D, and 3D nanostructures for efficient point-of-care biosensing.Biosensors & bioelectronics: X · 2022Article
- Review
- A colorimetric aptasensor based on gold nanoparticles for detection of microbial toxins: an alternative approach to conventional methods.Analytical and bioanalytical chemistry · 2022Review
- Recent Advances in Electrochemical Biosensors for the Detection ofBiosensors · 2022Review
- Review
- Article
- Molecular Diagnostic of Ochratoxin A With Specific Aptamers in Corn and Groundnut via Fabrication of a Microfluidic Device.Frontiers in nutrition · 2022Article
- Recent advances in nanomaterials integrated immunosensors for food toxin detection.Journal of food science and technology · 2022Review
- Label-free detection of SARS-CoV-2 Spike S1 antigen triggered by electroactive gold nanoparticles on antibody coated fluorine-doped tin oxide (FTO) electrode.Analytica chimica acta · 2021Article
- A Simple and Ultrasensitive Colorimetric Biosensor for Anatoxin-a Based on Aptamer and Gold Nanoparticles.Micromachines · 2021Article
- Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods.Journal of animal science and biotechnology · 2021Review
- Disposable Paper-Based Biosensors for the Point-of-Care Detection of Hazardous Contaminations-A Review.Biosensors · 2021Review
- Visual detection of tropomyosin, a major shrimp allergenic protein using gold nanoparticles (AuNPs)-assisted colorimetric aptasensor.Marine life science & technology · 2021Article
- Recent Advances in Microfluidic Devices for Contamination Detection and Quality Inspection of Milk.Micromachines · 2021Review
- Increasing the packing density of assays in paper-based microfluidic devices.Biomicrofluidics · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Contamination of milk by mycotoxins is a serious problem worldwide. Herein, we focused on the detection of aflatoxin B1 (AflB1) using a paper microfluidic device fabricated with specific aptamers as biorecognition elements. The fabrication process resulted in the generation of a leak proof microfluidic device where two zones were designed: control and test. Detection is achieved by color change when aflatoxin reacts with an aptamer followed by salt induced aggregation of gold nanoparticles. Specific aptamers for aflatoxin B1 were immobilized successfully onto the surface of gold nanoparticles. Biophysical characterization of the conjugated AuNPs-aptamer was done by UV-vis spectroscopy, DLS (dynamic light scattering), TEM (transmission electron microscopy). Under optimal conditions, the microfluidic device showed an excellent response for aflatoxin B1 detection in the range of 1 pM to 1 μM with a detection limit of up to 10 nM in spiked samples. Disadvantages associated with conventional techniques of ELISA were overcome by this one step detection technique with low operation cost, simple instrumentation, and user-friendly format with no interference due to chromatographic separation. The developed microfluidic paper-based analytical device (μPAD) can provide a tool for on-site detection of food toxins in less than a minute which is the main requirement for both qualitative and quantitative analysis in food safety and environmental monitoring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.