Evidence map›Paper›PMID 35496112›Full record

ArticleRSC advances2020

Stable immobilization of aldehyde ketone reductase mutants containing nonstandard amino acids on an epoxy resin

Huimin Li, Youcheng Yin, Anming Wang, Ningning Li, Ru Wang, Jing Zhang, Xinxin Chen, Xiaolin Pei, Tian Xie

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Non-canonical amino acids as a tool for the thermal stabilization of enzymes.Protein engineering, design & selection : PEDS · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Huimin LiCollege of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University Hangzhou 310014 P. R. China waming@hznu.edu.cn.
Youcheng YinHolistic Integrative Pharmacy Institutes, College of Medicine, Hangzhou Normal University Hangzhou Zhejiang China xbs@hznu.edu.cn.
Anming WangCollege of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University Hangzhou 310014 P. R. China waming@hznu.edu.cn.ORCID https://orcid.org/0000-0002-8480-5783
Ningning LiCollege of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University Hangzhou 310014 P. R. China waming@hznu.edu.cn.
Ru WangHolistic Integrative Pharmacy Institutes, College of Medicine, Hangzhou Normal University Hangzhou Zhejiang China xbs@hznu.edu.cn.
Jing ZhangCollege of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University Hangzhou 310014 P. R. China waming@hznu.edu.cn.
Xinxin ChenCollege of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University Hangzhou 310014 P. R. China waming@hznu.edu.cn.
Xiaolin PeiCollege of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University Hangzhou 310014 P. R. China waming@hznu.edu.cn.ORCID https://orcid.org/0000-0002-1953-4323
Tian XieHolistic Integrative Pharmacy Institutes, College of Medicine, Hangzhou Normal University Hangzhou Zhejiang China xbs@hznu.edu.cn.
Hangzhou Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To avoid random chemical linkage and achieve precisely directed immobilization, mutant enzymes were obtained and immobilized using an incorporated reactive nonstandard amino acid (NSAA). For this purpose, aldehyde ketone reductase (AKR) was used as a model enzyme, and 110Y, 114Y, 143Y, 162Q and 189Q were each replaced with

Identifiers

PMID35496112
PMCPMC9049136
OpenAlexW2999459105

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.