ArticleFrontiers in microbiology2022
Metagenomics Insights Into the Microbial Diversity and Microbiome Network Analysis on the Heterogeneity of Influent to Effluent Water.
Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Exploring community-level gut health amidst COVID-19 pandemic: a proposed application of longitudinal wastewater surveillance.Environmental science and pollution research international · 2026Article
- Comprehensive genome analysis of MDR Klebsiella pneumoniae in influent and effluent of a selected wastewater treatment plant.Scientific reports · 2025Article
- Wounds and the Microbiota: The Healing Interplay Between Host and Microbial Communities.International journal of molecular sciences · 2025Review
- Integrating Metagenomic and Culture-Based Techniques to Detect Foodborne Pathogens and Antimicrobial Resistance Genes in Malaysian Produce.Foods (Basel, Switzerland) · 2025Article
- Article
- Molecular Identification of Human and Plant Pathogens in Municipal Domestic Wastewater for Hydroponic System Applications.International journal of microbiology · 2025Article
- Article
- Metagenomic Analysis of Sediment Bacterial Diversity and Composition in Natural Lakes and Artificial Waterpoints of Tabuk Region in King Salman Bin Abdulaziz Royal Natural Reserve, Saudi Arabia.Life (Basel, Switzerland) · 2024Article
- Bacterial hosts of clinically significant beta-lactamase genes in Croatian wastewaters.FEMS microbiology ecology · 2024Article
- Anthelmintic resistance in soil-transmitted helminths: One-Health considerations.Parasitology research · 2023Review
- Wastewater microbial diversity versus molecular analysis at a glance: a mini-review.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Review
- Social demographics determinants for resistome and microbiome variation of a multiethnic community in Southern Malaysia.NPJ biofilms and microbiomes · 2023Article
- High-resolution characterization of short-term temporal variability in the taxonomic and resistome composition of wastewater influent.Microbial genomics · 2023Article
- Shotgun Metagenomic Analyses of Microbial Assemblages in the Aquatic Ecosystem of Winam Gulf of Lake Victoria, Kenya Reveals Multiclass Pollution.BioMed research international · 2023Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sanitizing the water sources of local communities is important to control the spread of microbial resistance genes, especially those for water-borne illnesses. The activities of antibiotic resistance gene (ARG)-host pathogens pose a threat to public health, and it has been estimated that the infection will lead up to 10 million deaths globally by the year 2050. Hence, in this study, we aim to analyze the efficiency of our municipal wastewater treatment plant (WWTP) process in producing pathogen-free water by investigating the microbial composition between influent and effluent water sites. Shotgun metagenomics sequencing using the Illumina platform was performed on the influent and effluent samples of six different WWTP sites located in Johore, Malaysia. After raw data pre-processing, the non-redundant contigs library was then aligned against BLASTP for taxonomy profiling and the Comprehensive Antibiotic Resistance Database for ARG annotation. Interestingly, the alpha-diversity result reported that effluent site samples showed higher abundance and diverse heterogeneity compared to the influent site. The principal component analysis (PCA) and non-metric multidimensional scaling (NMDS) plots also suggested that effluent sites showed high variation in the genetic material due to loosely clustered sample plots, as compared to the tightly clustered influent samples. This study has successfully identified the top three abundant phyla in influent-
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