ArticleApplications in plant sciences
Step-by-step protocol for the isolation and transient transformation of hornwort protoplasts.
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A rapid and efficient in planta assay for assessment of RNA editing factor performance.The Plant journal : for cell and molecular biology · 2026Article
- Genetic transformation and CRISPR/Cas12a-mediated gene editing of European beech (Fagus sylvatica L.) employing a transient protoplast system.Communications biology · 2026Article
- Establishment of Transient Transformation Systems in Welsh Onion (Plants (Basel, Switzerland) · 2025Article
- Pan-phylum genomes of hornworts reveal conserved autosomes but dynamic accessory and sex chromosomes.Nature plants · 2025Article
- An optimized transformation protocol for Anthoceros agrestis and three more hornwort species.The Plant journal : for cell and molecular biology · 2023Article
- Unique opportunities for future research on the alternative oxidase of plants.Plant physiology · 2023Article
- What can hornworts teach us?Frontiers in plant science · 2023Review
- An integrated physiology, cytology, and proteomics analysis reveals a network of sugarcane protoplast responses to enzymolysis.Frontiers in plant science · 2022Article
- Step-by-step protocol for the isolation and transient transformation of hornwort protoplasts.Applications in plant sciencesArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Premise: A detailed protocol for the protoplast transformation of hornwort tissue is not yet available, limiting molecular biological investigations of these plants and comparative analyses with other bryophytes, which display a gametophyte-dominant life cycle and are critical to understanding the evolution of key land plant traits. Methods and Results: We describe a detailed protocol to isolate and transiently transform protoplasts of the model hornwort Conclusions: This protocol represents a straightforward method for isolating and transforming
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Registered trials
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