ArticleApplications in plant sciences
Integrating tissue-direct PCR into genetic identification: An upgraded molecular ecology approach to survey fern gametophytes in the field.
Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 10 citations in OpenAlex.
- Fern Gametophytes Exhibit Distinct Patterns of Niche Expansion and Convergence in Ecophysiological Functioning.Ecology and evolution · 2026Article
- A DNA barcode reference of Asian ferns with expert-identified voucher specimens and DNA samples.Scientific data · 2024Article
- Systematics and Plastome Evolution in Schizaeaceae.Frontiers in plant science · 2022Article
- Two new species in the fern genusPhytoKeys · 2021Article
- The ecology and physiology of fern gametophytes: A methodological synthesis.Applications in plant sciencesArticle
- Identifying cryptic fern gametophytes using DNA barcoding: A review.Applications in plant sciencesArticle
- Integrating tissue-direct PCR into genetic identification: An upgraded molecular ecology approach to survey fern gametophytes in the field.Applications in plant sciencesArticle
- Providing the missing links in fern life history: Insights from a phenological survey of the gametophyte stage.Applications in plant sciencesArticle
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Premise: The gametophytes of different fern species collected in the field can be difficult to distinguish because of their morphological similarities. Nonetheless, emerging molecular ecology techniques are starting to be used to tackle such limitations. Here, using case studies and a detailed protocol, we demonstrate a convenient methodology, tissue-direct PCR (TD-PCR), that foregoes a traditional DNA extraction and facilitates the identification of fern gametophytes, as well as enabling the elucidation of their natural distribution. Methods: Based on updated plastome information, we designed a universal primer set targeting the Results: Compared with approaches requiring DNA extraction, the use of TD-PCR with either universal or taxon-specific primers could save significant time, money, labor, and research materials in the genetic identification of fern gametophytes. Discussion: The use of modern genetic tools can aid in the identification of fern gametophytes. An updated TD-PCR strategy not only facilitates the DNA-based identification of gametophytes, but also promotes new avenues of research for investigating these plants in the field.
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