ArticleThe EMBO journal1986
Molecular characterization of a karyophilic, histone-binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis.
Article in The EMBO journal, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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63 citing papers in PubMed.
- Making Mitotic Chromosomes in a Test Tube.Epigenomes · 2022Review
- NASP maintains histone H3-H4 homeostasis through two distinct H3 binding modes.Nucleic acids research · 2022Article
- The histone chaperone Nrp1 is required for chromatin stability and nuclear division in Tetrahymena thermophila.Epigenetics & chromatin · 2021Article
- Protein phosphorylation during Plasmodium berghei gametogenesis.Experimental parasitology · 2015Article
- Article
- The human histone chaperone sNASP interacts with linker and core histones through distinct mechanisms.Nucleic acids research · 2012Article
- Crystal structure and function of human nucleoplasmin (npm2): a histone chaperone in oocytes and embryos.Biochemistry · 2011Article
- Nucleosome formation activity of human somatic nuclear autoantigenic sperm protein (sNASP).The Journal of biological chemistry · 2010Article
- Identification of distinct SET/TAF-Ibeta domains required for core histone binding and quantitative characterisation of the interaction.BMC biochemistry · 2009Article
- Expanded binding specificity of the human histone chaperone NASP.Nucleic acids research · 2008Article
- sNASP, a histone H1-specific eukaryotic chaperone dimer that facilitates chromatin assembly.Biophysical journal · 2008Article
- Tpt1 activates transcription of oct4 and nanog in transplanted somatic nuclei.Current biology : CB · 2007Article
- Long-term evolution and functional diversification in the members of the nucleophosmin/nucleoplasmin family of nuclear chaperones.Genetics · 2006Article
- The characterization of amphibian nucleoplasmins yields new insight into their role in sperm chromatin remodeling.BMC genomics · 2006Article
- A novel karyoskeletal protein: characterization of protein NO145, the major component of nucleolar cortical skeleton in Xenopus oocytes.Molecular biology of the cell · 2001Article
- Dimerization of the largest subunit of chromatin assembly factor 1: importance in vitro and during Xenopus early development.The EMBO journal · 2001Article
- Cloning, expression and nuclear localization of human NPM3, a member of the nucleophosmin/nucleoplasmin family of nuclear chaperones.BMC genomics · 2001Article
- Gadd45, a p53-responsive stress protein, modifies DNA accessibility on damaged chromatin.Molecular and cellular biology · 1999Article
- Prothymosin alpha modulates the interaction of histone H1 with chromatin.Nucleic acids research · 1998Article
- Human and rat testis express two mRNA species encoding variants of NRD convertase, a metalloendopeptidase of the insulinase family.The Biochemical journal · 1997Article
3 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
In the amphibian oocyte, most of the non-chromatin-bound histones are not free but form complexes with specific karyophilic proteins, the most prominent being nucleoplasmin and 'protein N1/N2'. Using antibodies against polypeptide N1 and N2 (Mr approximately 105,000 and approximately 110,000) we have isolated, from a Xenopus laevis ovary lambda gt11 expression library, several full length cDNA clones encoding one of the two closely related polypeptides N1 and N2 (these could not be distinguished by hybridization techniques). The amino acid sequence deduced from one of these clones (N1/N2, lambda 106.2) defines a polypeptide of mol. wt 64,774. The remarkably high difference between the value of Mr approximately 110,000 estimated from SDS-PAGE mobility and the true mol. wt has been found for (i) the cell protein, (ii) the polypeptide synthesized in vitro by transcription and translation and (iii) the fusion protein with beta-galactosidase expressed in Escherichia coli, indicating that the protein runs anomalously on SDS-PAGE. The amino and carboxy termini of the purified protein N1/N2 have been confirmed by direct amino acid sequencing of CNBr fragments. The amino acid sequence displays two glutamic acid-rich domains, which are probably involved in the interaction with the histones, and a putative nuclear targeting signal with high homology to that of the SV40 large T-antigen which is located near the carboxy terminus.(ABSTRACT TRUNCATED AT 250 WORDS)
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