Evidence map›Paper›PMID 35492043›Full record

ArticleHardwareX2021

The incubot: A 3D printer-based microscope for long-term live cell imaging within a tissue culture incubator.

George O T Merces, Conor Kennedy, Blanca Lenoci, Emmanuel G Reynaud, Niamh Burke, Mark Pickering

Open access · goldAbstract read
In one paragraph

Article in HardwareX, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
10.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 49 citations in OpenAlex.

  1. Article
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  4. COMethods and protocols · 2024
    Article
  5. EnderScope: a low-cost 3D printer-based scanning microscope for microplastic detection.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2024
    Article
  6. Article
  7. Article
  8. Article
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  12. A review on low-cost microscopes for Open Science.Microscopy research and technique · 2022
    Review
  13. Article
  14. Article
  15. TheRoyal Society open science · 2022
    Article
  16. Article
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

George O T MercesSchool of Medicine, University College Dublin, Co. Dublin, D04 V1W8, Ireland.
Conor KennedySchool of Medicine, University College Dublin, Co. Dublin, D04 V1W8, Ireland.
Blanca LenociSchool of Medicine, University College Dublin, Co. Dublin, D04 V1W8, Ireland.
Emmanuel G ReynaudSchool of Biomolecular and Biomedical Science, University College Dublin, Co. Dublin, D04 V1W8, Ireland.
Niamh BurkeSchool of Medicine, University College Dublin, Co. Dublin, D04 V1W8, Ireland.
Mark PickeringSchool of Medicine, University College Dublin, Co. Dublin, D04 V1W8, Ireland.
University College Dublin · IE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Commercial live cell imaging systems represent a large financial burden to research groups, while current open source incubator microscopy systems lack adaptability and are sometimes inadequate for complex imaging experimentation. We present here a low-cost microscope designed for inclusion within a conventional tissue culture incubator. The build is constructed using an entry level 3D printer as the basis for the motion control system, with Raspberry Pi imaging and software integration, allowing for reflected, oblique, and fluorescence imaging of live cell monolayers. The open source nature of the design is aimed to facilitate adaptation by both the community at large and by individual researchers/groups. The development of an adaptable and easy-to-use graphic user interface (GUI) allows for the scientist to be at the core of experimental design through simple modifications of the base GUI code, or generation of an entirely purpose-built script. This adaptability will allow scientists to adapt this equipment for their experimental needs, as opposed to designing experiments to fit their current equipment. The build can be constructed for a cost of roughly €1000 and thus serves as a low-cost and adaptable addition to the open source microscopy community.

Indexed as

Live-Cell ImagingLow-CostMicroscopyPythonRaspberry PiTissue Culture

Identifiers

PMID35492043
PMCPMC9041206
OpenAlexW3138049415

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.