Evidence map›Paper›PMID 35490489›Full record

ArticleResearch in veterinary science2022

Exploring the association of intratumoral immune cell infiltrates with histopathologic grade in canine mast cell tumors.

Victoria R Costa, Aimee M Soileau, Chin-Chi Liu, Cambri E Moeller, Mariano Carossino, Ingeborg M Langohr, Sita S Withers

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Research in veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Victoria R CostaLouisiana State University, School of Veterinary Medicine, Department of Veterinary Clinical Sciences, 1909 Skip Bertman Dr., Baton Rouge, LA 70803, USA.
Aimee M SoileauLouisiana State University, School of Veterinary Medicine, Department of Veterinary Clinical Sciences, 1909 Skip Bertman Dr., Baton Rouge, LA 70803, USA.
Chin-Chi LiuLouisiana State University, School of Veterinary Medicine, Department of Veterinary Clinical Sciences, 1909 Skip Bertman Dr., Baton Rouge, LA 70803, USA.
Cambri E MoellerLouisiana State University, School of Veterinary Medicine, Department of Veterinary Clinical Sciences, 1909 Skip Bertman Dr., Baton Rouge, LA 70803, USA.
Mariano CarossinoLouisiana State University, Department of Pathobiological Sciences, 1909 Skip Bertman Dr., Baton Rouge, LA 70803, USA; Louisiana State University, Louisiana Animal Disease Diagnostic Laboratory (LADDL), River Rd, #1043, Baton Rouge, LA 70803, USA.
Ingeborg M LangohrLouisiana State University, Department of Pathobiological Sciences, 1909 Skip Bertman Dr., Baton Rouge, LA 70803, USA; Louisiana State University, Louisiana Animal Disease Diagnostic Laboratory (LADDL), River Rd, #1043, Baton Rouge, LA 70803, USA.
Sita S WithersLouisiana State University, School of Veterinary Medicine, Department of Veterinary Clinical Sciences, 1909 Skip Bertman Dr., Baton Rouge, LA 70803, USA. Electronic address: withers1@lsu.edu.
Louisiana State University · US

Funding

Strategic inhibition of EGFR-family signaling using novel peptidomimetic inhibitors of HER-2 for the treatment of osteosarcomaP20GM135000 · NIGMS · LOUISIANA STATE UNIV A&M COL BATON ROUGE · PI CHAMCHEU, JEAN CHRISTOPHER · 2021 to 2025
$11.3M
NIGMS NIH HHS P20 GM135000
6 · The paper itself

Abstract

Cutaneous canine mast cell tumors (ccMCTs) vary in their biological behavior, treatment, and prognosis, based on their grade. Immune cell infiltration has been associated with prognosis and response to treatments in some human cancers, and immune-targeting therapeutics are increasingly being explored in veterinary oncology. However, currently little is known about the tumor microenvironment (TME) in ccMCTs. Therefore, the objective of this study was to determine the prevalence of T lymphocytes, T regulatory lymphocytes, PD-1+ cells and macrophages in low- and high-grade ccMCTs. Thirty low-grade and 20 high-grade formalin-fixed paraffin-embedded ccMCT samples were included. Immunohistochemistry (IHC) was performed to detect CD3, FOXP3, Iba1, and PD-1 on sequential sections. Three 400x fields with the highest numbers of CD3+ cells were identified for each tumor. The percentage of CD3+, FOXP3+, and Iba1+ cells, and the number of PD-1+ cells, was quantified in each of these three "hot-spot" fields using ImageJ software. Iba1 expression was significantly greater in high-grade compared to low-grade ccMCTs (mean = 12.5% vs. 9.6%, p = 0.043). PD-1 expression was low overall, but a significantly higher number of PD-1-expressing cells was observed in high-grade ccMCTs (median 1 vs. 0, p = 0.001). No significant difference was noted in CD3 and FOXP3 expression between ccMCT grades. Macrophages and PD-1+ cells were more frequent in high-grade, compared to low-grade ccMCTs. Further studies are needed to define the role of macrophages and rare PD-1+ cells in high-grade ccMCTs.

Indexed as

Dog DiseasesNeoplasmsAnimalsB7-H1 AntigenDogsForkhead Transcription FactorsLymphocytes, Tumor-InfiltratingPrognosisProgrammed Cell Death 1 ReceptorTumor MicroenvironmentB7-H1 AntigenForkhead Transcription FactorsProgrammed Cell Death 1 ReceptorDogsImmune cellsMast cell tumorPD-1T-regulatory cellsTumor microenvironment

Identifiers

PMID35490489
PMCPMC11293894
OpenAlexW4224278703

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.