Evidence map›Paper›PMID 35487464›Full record

ArticleJournal of structural biology2022

Montage electron tomography of vitrified specimens.

Ariana Peck, Stephen D Carter, Huanghao Mai, Songye Chen, Alister Burt, Grant J Jensen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. Article
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  7. SLICK: A Sandwich-LIke Culturing Kit forbioRxiv : the preprint server for biology · 2025
    Article
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  19. Square beams for optimal tiling in TEM.bioRxiv : the preprint server for biology · 2023
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ariana PeckDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Stephen D CarterDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Huanghao MaiDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
Songye ChenDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.
Alister BurtScientific Computing Department, Science and Technology Facilities Council, Research Complex at Harwell, Didcot OX11 0FA, United Kingdom.
Grant J JensenDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; College of Physical and Mathematical Sciences, Brigham Young University, Provo, UT 84602, USA. Electronic address: grant_jensen@byu.edu.

Funding

Structure and Function of Pathogenesis-Associated Bacterial Structures by Electron CryotomographyR01AI127401 · NIAID · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI GRANT J JENSEN · 2016 to 2026
$3.9M
NIAID NIH HHS R01 AI127401
6 · The paper itself

Abstract

Cryo-electron tomography provides detailed views of macromolecules in situ. However, imaging a large field of view to provide more cellular context requires reducing magnification during data collection, which in turn restricts the resolution. To circumvent this trade-off between field of view and resolution, we have developed a montage data collection scheme that uniformly distributes the dose throughout the specimen. In this approach, sets of slightly overlapping circular tiles are collected at high magnification and stitched to form a composite projection image at each tilt angle. These montage tilt-series are then reconstructed into massive tomograms with a small pixel size but a large field of view. For proof-of-principle, we applied this method to the thin edge of HeLa cells. Thon rings to better than 10 Å were detected in the montaged tilt-series, and diverse cellular features were observed in the resulting tomograms. These results indicate that the additional dose required by this technique is not prohibitive to performing structural analysis to intermediate resolution across a large field of view. We anticipate that montage tomography will prove particularly useful for lamellae, increase the likelihood of imaging rare cellular events, and facilitate visual proteomics.

Indexed as

Electron Microscope TomographyImage Processing, Computer-AssistedCryoelectron MicroscopyHeLa CellsHumansMacromolecular SubstancesMacromolecular SubstancesCellular biologyCryo-electron microscopyImage processingMontage tomographyUltrastructural analysis

Identifiers

PMID35487464
PMCPMC10081539

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.