ArticlePloS one2022
A novel Fiji/ImageJ plugin for the rapid analysis of blebbing cells.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- CD24 Regulates the Formation of Ectosomes in B Lymphocytes.Journal of extracellular vesicles · 2025Article
- The activation of INF2 by Piezo1/CaCurrent biology : CB · 2025Article
- Structure of the F-tractin-F-actin complex.The Journal of cell biology · 2025Article
- The activation of INF2 by Piezo1/CabioRxiv : the preprint server for biology · 2024Article
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Authors and funding
3 authors.
Funding
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Abstract
When confined, cells have recently been shown to undergo a phenotypic switch to what has been termed, fast amoeboid (leader bleb-based) migration. However, as this is a nascent area of research, few tools are available for the rapid analysis of cell behavior. Here, we demonstrate that a novel Fiji/ImageJ-based plugin, Analyze_Blebs, can be used to quickly obtain cell migration parameters and morphometrics from time lapse images. As validation, we show that Analyze_Blebs can detect significant differences in cell migration and morphometrics, such as the largest bleb size, upon introducing different live markers of F-actin, including F-tractin and LifeAct tagged with green and red fluorescent proteins. We also demonstrate, using flow cytometry, that live markers increase total levels of F-actin. Furthermore, that F-tractin increases cell stiffness, which was found to correlate with a decrease in migration, thus reaffirming the importance of cell mechanics as a determinant of Leader Bleb-Based Migration (LBBM).
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