Evidence map›Paper›PMID 35486030›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022

Coupling Lipid Labeling and Click Chemistry Enables Isolation of Extracellular Vesicles for Noninvasive Detection of Oncogenic Gene Alterations.

Na Sun, Benjamin V Tran, Zishan Peng, Jing Wang, Ceng Zhang, Peng Yang, Tiffany X Zhang, Josephine Widjaja, Ryan Y Zhang, Wenxi Xia and 14 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.

  1. Pooled it
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  12. Extracellular Vesicle Preparation and Analysis: A State-of-the-Art Review.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors at 8 institutions in 3 countries.

Na SunCalifornia NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Benjamin V TranDepartment of Surgery, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Zishan PengDepartment of Pathology, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
Jing WangDepartment of Pathology, Shanghai Medical College, Fudan University, Shanghai, 200032, P. R. China.
Ceng ZhangCalifornia NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Peng YangCalifornia NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Tiffany X ZhangCalifornia NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Josephine WidjajaCalifornia NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Ryan Y ZhangCalifornia NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Wenxi XiaCalifornia NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Alexandra KeirDepartment of Pediatrics, David Geffen School of Medicine, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, and Children's Discovery and Innovation Institute, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Jia-Wei SheSmart Organic Materials Laboratory, Institute of Chemistry, Academia Sinica, Nankang, Taipei, 115, Taiwan.
Hsiao-Hua YuSmart Organic Materials Laboratory, Institute of Chemistry, Academia Sinica, Nankang, Taipei, 115, Taiwan.
Jing-Jong ShyueResearch Center for Applied Sciences, Academia Sinica, Nankang, Taipei, 115, Taiwan.
Hongguang ZhuDepartment of Pathology, Shanghai Medical College, Fudan University, Shanghai, 200032, P. R. China.
Vatche G AgopianDepartment of Surgery, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Renjun PeiKey Laboratory for Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Suzhou, 215123, P. R. China.
James S TomlinsonDepartment of Surgery, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Jeffrey A ToretskyDepartments of Oncology and Pediatrics, Georgetown University, Washington, DC, 20057, USA.
Steven J JonasDepartment of Pediatrics, David Geffen School of Medicine, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, and Children's Discovery and Innovation Institute, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Noah FedermanDepartment of Pediatrics, David Geffen School of Medicine, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, and Children's Discovery and Innovation Institute, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
Shaohua LuDepartment of Pathology, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China.
Hsian-Rong TsengCalifornia NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.ORCID 0000-0003-0942-5905
Yazhen ZhuCalifornia NanoSystems Institute, Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA, 90095, USA.
California NanoSystems Institute · USUniversity of California, Los Angeles · USInstitute of Sociology, Academia Sinica · TWShanghai Medical College of Fudan University · CNSun Yat-sen University · CNGeorgetown University · USResearch Center for Applied Science, Academia Sinica · TWSuzhou Institute of Nano-tech and Nano-bionics · CN

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
Non-Invasive Prenatal Diagnostics Based on Circulating TrophoblastsU01EB026421 · NIBIB · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PISARSKA, MARGARETA, TSENG, HSIAN-RONG · 2019 to 2023
$4.0M
HCC EV Digital Scoring Assay for assessing treatment response in HCC patientsR01CA253651 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2020 to 2024
$3.3M
Extracellular Vesicle-Based Digital Scoring Assay for Detecting Early-stage Hepatocellular CarcinomaR01CA255727 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHU, YAZHEN · 2021 to 2025
$3.2M
Integrated analysis of HCC CTCs for Liver Transplant Candidate SelectionR01CA246304 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2020 to 2024
$2.8M
Thermoresponsive NanoVelcro CTC Purification System for Prostate Cancer ProfilingU01CA198900 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI POSADAS, EDWIN MELENCIO, TSENG, HSIAN-RONG · 2015 to 2019
$2.4M
Dissection of EWS-FLI1 oncogenic mechanisms and small molecule targetingR01CA233619 · NCI · GEORGETOWN UNIVERSITY · PI TORETSKY, JEFFREY A · 2020 to 2025
$1.9M
Click Chemistry-Mediated Microfluidic Sorting for HCC CTCsR21CA235340 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2019 to 2021
$543k
Biomimetic NanoVilli Chips for Extracellular Vesicles in Hepatocellular CarcinomaR21CA240887 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI AGOPIAN, VATCHE, TSENG, HSIAN-RONG · 2020 to 2021
$419k
NCI NIH HHS P30 CA016042NCI NIH HHS R01 CA233619NCI NIH HHS R01 CA246304NCI NIH HHS R01 CA253651NCI NIH HHS R01 CA255727NCI NIH HHS R21 CA235340NCI NIH HHS R21 CA240887NCI NIH HHS U01 CA198900NIBIB NIH HHS U01 EB026421NIH HHS R01 CA246304NIH HHS R01 CA253651NIH HHS R01 CA255727NIH HHS R21 CA235340NIH HHS R21 CA240887NIH HHS U01 CA198900NIH HHS U01 EB026421
6 · The paper itself

Abstract

Well-preserved molecular cargo in circulating extracellular vesicles (EVs) offers an ideal material for detecting oncogenic gene alterations in cancer patients, providing a noninvasive diagnostic solution for detection of disease status and monitoring treatment response. Therefore, technologies that conveniently isolate EVs with sufficient efficiency are desperately needed. Here, a lipid labeling and click chemistry-based EV capture platform ("Click Beads"), which is ideal for EV message ribonucleic acid (mRNA) assays due to its efficient, convenient, and rapid purification of EVs, enabling downstream molecular quantification using reverse transcription digital polymerase chain reaction (RT-dPCR) is described and demonstrated. Ewing sarcoma protein (EWS) gene rearrangements and kirsten rat sarcoma viral oncogene homolog (KRAS) gene mutation status are detected and quantified using EVs isolated by Click Beads and matched with those identified in biopsy specimens from Ewing sarcoma or pancreatic cancer patients. Moreover, the quantification of gene alterations can be used for monitoring treatment responses and disease progression.

Indexed as

Extracellular VesiclesSarcoma, EwingCarcinogenesisClick ChemistryGenes, rasHumansLipidsRNA-Binding Protein EWSLipidsRNA-Binding Protein EWSclick chemistryextracellular vesiclesgene alteration quantificationlipid labeling

Identifiers

PMID35486030
PMCPMC9108594
OpenAlexW4221079066

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.