ArticleNature plants2022
A conformational switch in the SCF-D3/MAX2 ubiquitin ligase facilitates strigolactone signalling.
Article in Nature plants, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 70 citations in OpenAlex.
- Strigolactone-mediated architecture regulation and stress resilience: Insights and innovations for crop breeding.Journal of integrative plant biology · 2026Review
- The F-Box Protein DWARF1 Controls Plant Height by Modulating MtSMXL6 and MtSMXL7 Stability in Medicago truncatula.Plant, cell & environment · 2026Article
- Towards an integrated molecular understanding of plant hormones.Journal of integrative plant biology · 2026Review
- Structure-informed engineering of plant-microbe interactions.The Plant journal : for cell and molecular biology · 2026Review
- Citric acid alters Arabidopsis root morphology and development through ROS-dependent and ROS-independent mechanisms.Plant physiology · 2026Article
- Enhanced Polar Auxin Transport and Reduced Brassinosteroid Activity Drive Internode Elongation in Chinese Fir (Plants (Basel, Switzerland) · 2026Article
- Salicylic acid modulates its catabolic enzymes via proteasomal degradation linked to SCF-associated proximity networks.Nature communications · 2026Article
- Decoding the sugar-strigolactone crosstalk: new frontier in plant growth and stress resilience.Horticulture research · 2026Article
- Genome wide association mapping and haplotype analysis identify genetic loci and candidate genes associated with yield related traits in rice (Frontiers in plant science · 2026Article
- A conserved structural framework accompanies evolutionary divergence and expression specialization in the grapevine SKP1 family.Frontiers in plant science · 2026Article
- Dynamic ASK1 proximity networks uncover SCF-dependent and noncanonical roles in ABA and drought adaptation.bioRxiv : the preprint server for biology · 2025Article
- Trehalose 6-phosphate - a central regulator at the crossroads of sugar signalling, metabolism, and development.The New phytologist · 2025Review
- In vitro dynamic and quantitative monitoring of strigolactone-signaling complex formation by time-resolved FRET.The Plant journal : for cell and molecular biology · 2025Article
- Mechanism of cooperative strigolactone perception by the MAX2 ubiquitin ligase-receptor-substrate complex.Nature communications · 2025Article
- An N-terminal domain specifies developmental control by the SMAX1-LIKE family of transcriptional regulators inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Multifaceted Impact of Karrikin Signaling in Plants.International journal of molecular sciences · 2025Review
- Structural requirements of KAI2 ligands for activation of signal transduction.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Strigolactone and karrikin receptors regulate phytohormone biosynthetic and catabolic processes.Plant cell reports · 2025Article
- The Role of E3 Ubiquitin Ligase GeneInternational journal of molecular sciences · 2025Review
- Cotton GhMAX2 promotes single-celled fiber elongation by releasing the GhS1FA-mediated inhibition of fatty acid biosynthesis.Plant cell reports · 2025Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strigolactones (SLs) are a class of plant hormones that regulate numerous processes of growth and development. SL perception and signal activation involves interaction between F-box E3 ubiquitin ligase D3/MAX2 and DWARF14 (D14) α/β-hydrolase in a SL-dependent manner and targeting of D53/SMXL6/7/8 transcriptional repressors (SMXLs) for proteasome-mediated degradation. D3/MAX2 has been shown to exist in multiple conformational states in which the C-terminal helix (CTH) undergoes a closed-to-open dynamics and regulates D14 binding and SL perception. Despite the multiple modes of D3-D14 interactions found in vitro, the residues that regulate the conformational switch of D3/MAX2 CTH in targeting D53/SMXLs and the subsequent effect on SL signalling remain unclear. Here we elucidate the functional dynamics of ASK1-D3/MAX2 in SL signalling by leveraging conformational switch mutants in vitro and in plants. We report the crystal structure of a dislodged CTH of the ASK1-D3 mutant and demonstrate that disruptions in CTH plasticity via either CRISPR-Cas9 genome editing or expression of point mutation mutants result in impairment of SL signalling. We show that the conformational switch in ASK1-D3/MAX2 CTH directly regulates ubiquitin-mediated protein degradation. A dislodged conformation involved in D53/SMXLs SL-dependent recruitment and ubiquitination and an engaged conformation are required for the release of polyubiquitinated D53/SMXLs and subsequently D14 for proteasomal degradation. Finally, we uncovered an organic acid metabolite that can directly trigger the D3/MAX2 CTH conformational switch. Our findings unravel a new regulatory function of a SKP1-CUL1-F-box ubiquitin ligase in plant signalling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.