ArticleNature communications2022
Evanescent scattering imaging of single protein binding kinetics and DNA conformation changes.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- Twist-encoded magnetic plasmon interferometry for label-free, orientation-resolved single-molecule imaging.Science advances · 2026Article
- Surface Plasmon Resonance Imaging and Microscopy Modalities for Information-Rich, Label-Free Analysis of Biomolecular Interactions and Disease Biomarkers.Chemical & biomedical imaging · 2026Review
- Evaluating Antibody Quality via Simultaneous Size and Charge Measurement with Single Protein Oscillators.Analytical chemistry · 2026Article
- Article
- IR-AMES uncovers structure and composition of Alzheimer's tau oligomers.bioRxiv : the preprint server for biology · 2026Article
- Label-free mass and size characterization of few-kDa biomolecules by hierarchical vision transformer augmented nanofluidic scattering microscopy.Nature communications · 2026Article
- Meta-amplified dark-field interferometric scattering microscopy.Nature communications · 2026Article
- Dark-Field Scattering Imaging and Spectroscopy.ACS applied materials & interfaces · 2025Review
- Multiparametric Characterization of Individual Suspended Nanoparticles Using Confocal Fluorescence and Interferometric Scattering Microscopy with Microfluidic Confinement.Nano letters · 2025Article
- Quantitative, Label-Free Mapping of Cell Force Dynamics.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Label-Free Single-Molecule Immunoassay.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Large field-of-view plasmonic scattering imaging and sensing of nanoparticles with isotropic point-spread-function.Nature communications · 2025Article
- Enhanced Interferometric Imaging by Rotating Coherent Scattering Microscopy.ACS photonics · 2025Article
- Review
- Single molecule-driven nanomotors reveal the dynamic-disordered chemomechanical transduction of active enzymes.Science advances · 2025Article
- In Situ and Label-Free Quantification of Membrane Protein-Ligand Interactions Using Optical Imaging Techniques: A Review.Biosensors · 2024Review
- Dielectric Surface-Based Biosensors for Enhanced Detection of Biomolecular Interactions: Advances and Applications.Biosensors · 2024Review
- Article
- Label-Free Optical Imaging of Nanoscale Single Entities.ACS sensors · 2024Review
- Label-Free Tracking of Proteins through Plasmon-Enhanced Interference.ACS nanoscience Au · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Evanescent illumination has been widely used to detect single biological macromolecules because it can notably enhance light-analyte interaction. However, the current evanescent single-molecule detection system usually requires specially designed microspheres or nanomaterials. Here we show that single protein detection and imaging can be realized on a plain glass surface by imaging the interference between the evanescent lights scattered by the single proteins and by the natural roughness of the cover glass. This allows us to quantify the sizes of single proteins, characterize the protein-antibody interactions at the single-molecule level, and analyze the heterogeneity of single protein binding behaviors. In addition, owing to the exponential distribution of evanescent field intensity, the evanescent imaging system can track the analyte axial movement with high resolution, which can be used to analyze the DNA conformation changes, providing one solution for detecting small molecules, such as microRNA. This work demonstrates a label-free single protein imaging method with ordinary consumables and may pave a road for detecting small biological molecules.
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Registered trials
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