ArticleJournal of neuroscience methods2022
Measuring metabolic rate in single flies during sleep and waking states via indirect calorimetry.
Article in Journal of neuroscience methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Age-dependent physiological responses and longevity in three Drosophila species with contrasting lifespans exposed to low temperature and constant darkness.Biogerontology · 2026Article
- Peripheral metabolic dysfunction drives sleep disruption in TDP-43 proteinopathy.bioRxiv : the preprint server for biology · 2026Article
- Regulation of Metabolic Rhythms by Glial Clocks.Journal of biological rhythms · 2026Article
- Sleep rescues age-associated loss of glial engulfment.PLoS genetics · 2026Article
- Energy Deficit Is a Key Driver of Sleep Homeostasis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Early life sleep is associated with longevity inbioRxiv : the preprint server for biology · 2025Article
- Oxygen consumption rate of flatworms under the influence of wake- and sleep-promoting neurotransmitters.Journal of experimental zoology. Part A, Ecological and integrative physiology · 2024Article
- An electrophysiological correlate of sleep in a shark.Journal of experimental zoology. Part A, Ecological and integrative physiology · 2024Article
- Neurofibromin 1 mediates sleep depth in Drosophila.PLoS genetics · 2023Article
- Article
- Measuring O2 Consumption in Drosophila Melanogaster using Coulometric Microrespirometry.Journal of visualized experiments : JoVE · 2023Article
- Article
- Article
- Spenito-dependent metabolic sexual dimorphism intrinsic to fat storage cells.bioRxiv : the preprint server for biology · 2023Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDrosophila melanogaster is a leading genetic model for studying the neural regulation of sleep. Sleep is associated with changes in behavior and physiological state that are largely conserved across species. The investigation of sleep in flies has predominantly focused on behavioral readouts of sleep because physiological measurements, including changes in brain activity and metabolic rate, are less accessible. We have previously used stop-flow indirect calorimetry to measure whole body metabolic rate in single flies and have shown that in flies, like mammals, metabolic rate is reduced during sleep. NEW
methodHere, we describe a modified version of this system that allows for efficient and highly sensitive acquisition of CO
resultsIn this modified system, we show that sleep-dependent changes in metabolic rate are diminished in aging flies, supporting the notion that sleep quality is reduced as flies age. We also describe a modification that allows for simultaneous acquisition of CO COMPARISON WITH EXISTING METHODS AND
conclusionsTaken together, the measurement of metabolic rate via indirect calorimetry not only provides a physiological readout of sleep depth, but also provides insight the metabolic regulation of nutrient utilization, with broad applications to genetic studies in flies.
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