Evidence map›Paper›PMID 35482202›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays.

Elliot Corless, Yining Hao, Huan Jia, Patthara Kongsuphol, Dousabel M Y Tay, Say Yong Ng, Hadley D Sikes

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Elliot CorlessDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID 0000-0002-5033-0601
Yining HaoDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID 0000-0002-4066-048X
Huan JiaAntimicrobial Resistance Interdisciplinary Research Group (AMR-IRG), Singapore-MIT Alliance in Research and Technology (SMART), Singapore, Singapore.ORCID 0000-0002-8091-8932
Patthara KongsupholAntimicrobial Resistance Interdisciplinary Research Group (AMR-IRG), Singapore-MIT Alliance in Research and Technology (SMART), Singapore, Singapore.ORCID 0000-0003-0336-1672
Dousabel M Y TayDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Say Yong NgAntimicrobial Resistance Interdisciplinary Research Group (AMR-IRG), Singapore-MIT Alliance in Research and Technology (SMART), Singapore, Singapore.
Hadley D SikesDepartment of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. sikes@mit.edu.ORCID 0000-0002-7096-138X
Singapore-MIT Alliance for Research and Technology · SGMassachusetts Institute of Technology · US

Funding

Acquisition of Octet Biolayer Interferometry system for MIT biophysics facilityS10OD016326 · OD · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI IMPERIALI, BARBARA · 2014 to 2014
$180k
NIH HHS S10 OD016326
6 · The paper itself

Abstract

Many point-of-care diagnostic tests rely on a pair of monoclonal antibodies that bind to two distinct epitopes of a molecule of interest. This protocol describes the identification and generation of such affinity pairs based on an easily produced small protein scaffold rcSso7d which can substitute monoclonal antibodies. These strong binding variants are identified from a large yeast display library. The approach described can be significantly faster than antibody generation and epitope binning, yielding affinity pairs synthesized in common bacterial protein synthesis strains, enabling the rapid generation of novel diagnostic tools.

Indexed as

Antibodies, MonoclonalEpitopesGene LibraryImmunoassayAntibodies, MonoclonalEpitopesAffinity pairsAntigensBiomarkersBiosensorEngineered proteinsImmunoassaysPaper-based diagnosticsrcSso7dReagentsThermophileYeast surface display

Identifiers

PMID35482202
OpenAlexW4225562145

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.