Evidence map›Paper›PMID 35482195›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology.

Sara Linciano, Ee Lin Wong, Ylenia Mazzocato, Monica Chinellato, Tiziano Scaravetti, Alberto Caregnato, Veronica Cacco, Zhanna Romanyuk, Alessandro Angelini

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Sara LincianoDepartment of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy.
Ee Lin WongDepartment of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy.
Ylenia MazzocatoDepartment of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy.
Monica ChinellatoDepartment of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy.
Tiziano ScaravettiDepartment of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy.
Alberto CaregnatoDepartment of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy.
Veronica CaccoDepartment of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy.
Zhanna RomanyukDepartment of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy.
Alessandro AngeliniDepartment of Molecular Sciences and Nanosystems, Ca' Foscari University of Venice, Mestre, Italy. alessandro.angelini@unive.it.
Ca' Foscari University of Venice · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ability of cross-reactive antibodies to bind multiple related or unrelated targets derived from different species provides not only superior therapeutic efficacy but also a better assessment of treatment toxicity, thereby facilitating the transition from preclinical models to human clinical studies. This chapter provides some guidelines for the directed evolution of cross-reactive antibodies using yeast surface display technology. Cross-reactive antibodies are initially isolated from a naïve library by combining highly avid magnetic bead separations followed by multiple cycles of flow cytometry sorting. Once initial cross-reactive clones are identified, sequential rounds of mutagenesis and two-pressure selection strategies are applied to engineer cross-reactive antibodies with improved affinity and yet retained or superior cross-reactivity.

Indexed as

AntibodiesSaccharomyces cerevisiaeCross ReactionsGene LibraryHumansTechnologyAntibodiesAntibody engineeringCombinatorial library screeningCross-reactivityDirected evolutionMultispecificityPromiscuityProtein recognitionYeast surface display

Identifiers

PMID35482195
OpenAlexW4225726195

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.