Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.

Benjamin J Umlauf, John S Kuo, Eric V Shusta

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Co-cultures of cerebellar slices from mice with differentF1000Research · 2022
Article

4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Benjamin J UmlaufDepartment of Neurosurgery, Dell Medical School, The University of Texas at Austin, Austin, TX, USA. benjamin.umlauf@austin.utexas.edu.

John S KuoDepartment of Neurosurgery, Dell Medical School, The University of Texas at Austin, Austin, TX, USA.

Eric V ShustaDepartments of Chemical and Biological Engineering and Neurological Surgery, University of Wisconsin-Madison, Madison, WI, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells. This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases. Certain components of ECM are ubiquitous among all tissue; however, each biological tissue also displays unique variations that can be identified using biopanning techniques. Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface. Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface. These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.

Indexed as

Extracellular MatrixSaccharomyces cerevisiaeAnimalsBrainCollagenLymphocytesMiceCollagenBiopanningBlood–brain barrierExtracellular matrixNeuropathologiesVariable lymphocyte receptor (VLR)Yeast surface display

Identifiers

PMID35482194

DOI10.1007/978-1-4939-2748-7_4

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.