Evidence map›Paper›PMID 35482183›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation.

Byong H Kang, Brianna M Lax, K Dane Wittrup

Erratum issuedAbstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Both intratumoral regulatory T cell depletion and CTLA-4 antagonism are required for maximum efficacy of anti-CTLA-4 antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Byong H Kang *Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Brianna M Lax *Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
K Dane WittrupDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. wittrup@mit.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Yeast surface display is a powerful directed evolution method for developing and engineering protein molecules to attain desired properties. Here, updated protocols are presented for purposes of identification of lead binders and their affinity maturation. Large libraries are screened by magnetic bead selections followed by flow cytometric selections. Upon identification and characterization of single clones, their affinities are improved by an iterative process of mutagenesis and fluorescence-activated cell sorting.

Indexed as

Protein EngineeringSaccharomyces cerevisiaeFlow CytometryGene LibraryMutagenesisAffinity maturationDirected evolutionLibrary screeningProtein engineeringYeast surface display

Identifiers

PMID35482183

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.